Skip to main content
SpringerLink
Log in

Complete maps of IS1, IS2, IS3, IS4, IS5, IS30 and IS150 locations in Escherichia coli K12

  • Published:
Molecular and General Genetics MGG Aims and scope Submit manuscript

Summary

In this paper complete distribution maps are presented of the seven IS elements 1, 2, 3, 4, 5, 30 and 150. These maps were obtained during the construction of an almost complete restriction map of the Escherichia coli genome of K12 strain BHB2600. The positions of IS elements were correlated to this map. The distribution of integration sites of all IS types is nonrandom. Besides a large gap from 79 min to 96 min, there is a pronounced IS cluster at 6 min and another at 97 min, map locations that have low gene incidences on the classical map. One cluster coincides with a region of IS induced rearrangements. The IS distribution pattern was compared to patterns of strains W3110 and HB101.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Bachmann BJ (1983) Linkage map of E. coli K12, Edition 7. Microbiol Rev 47:180–230

    Google Scholar 

  • Bachmann BJ, Low KB (1980) Linkage map of E. coli K12, Edition 6. Microbiol Rev 44:1–56

    Google Scholar 

  • Barcellini-Couget S, Galucci P, Bouchè JP (1988) E. coli dicB operon includes a truncated IS2 element. Nucleic Acids Res 16:10388

    Google Scholar 

  • Besemer J, Herpers M (1977) Suppression of polarity of insertion mutations within the gal operon of E. coli. Mol Gen Genet 151:295–304

    Google Scholar 

  • Birkenbihl RP, Vielmetter W (1989) Cosmid-derived map of E. coli strain BHB2600 in comparison to the map of strain W3110. Nucleic Acids Res 17:5057–5069

    Google Scholar 

  • Blasband AJ, Marcotte Jr, Schnaitman CA (1986) Structure of the lc and nmpC outer membrane porin protein genes of lambdoid bacteriophage. J Biol Chem 261:12723–12732

    Google Scholar 

  • Botstein D, Shortle J (1985) Strategies and applications of in vitro mutagenesis. Science 229:1193–1201

    Google Scholar 

  • Boyer HW, Roulland-Dussoix D (1969) A complementation analysis of the restriction and modification of DNA in E. coli. J Mol Biol 41:459

    Google Scholar 

  • Calos MP, Miller JH (1980) Transposable elements. Cell 20:579–595

    Google Scholar 

  • Charlier D, Piette J, Glansdorff N (1982) IS3 can function as a mobile promotor in E. coli. Nucleic Acids Res 10: 5935–5948

    Google Scholar 

  • Dalrymple B, Caspers P, Arber W (1984) Nucleotide sequence of the procaryotic mobile genetic element IS30. EMBO J 3:2145–2149

    Google Scholar 

  • Engler JA, v. Bree MP (1981) The nucleotide sequence and protein-coding capability of the transposable element IS5. Gene 14:155–163

    Google Scholar 

  • Gamas P, Chandler MG, Prentki P, Galas DJ (1987) E. coli integration host factor binds specifically to the ends of the insertion sequence IS1 and to its major insertion hot spot in pBR322. J Mol Biol 195:261–272

    Google Scholar 

  • Ghosal D, Saedler H (1979) Nucleotide sequence of the transposable DNA-element IS2. Nucleic Acids Res 6:1111–1122

    Google Scholar 

  • Hadley RG, Ming H, Timmons M, Yun K, Deonier RC (1983) A partial restriction map of the proA-PurE region of the E. coli K12 genome. Gene 22:281–287

    Google Scholar 

  • Hill CW, Harnish BW (1981) Inversions between ribosomal RNA genes of E. coli. Proc Natl Acad Sci USA 78:7069–7072

    Google Scholar 

  • Holloway BW (1979) Plasmids that mobilize bacterial chromosome. Plasmid 2:1–19

    Google Scholar 

  • Hu S, Ptashne K, Cohen SN, Davidson N (1975) αβ Sequence of F is IS3. J Bacteriol 123:687–692

    Google Scholar 

  • Iida S, Meyer J, Arber W (1983) Procaryotic IS elements. In: Shapiro JA (ed) Mobile genetic elements. Academic Press, New York, pp 159–221

    Google Scholar 

  • Ish-Horowicz D, Burke JF (1981) Rapid and efficient cosmid cloning. Nucleic Acids Res 9:2989–2998

    Google Scholar 

  • Jaurin B, Normark S (1983) Insertion of IS2 creates a novel ampC promotor in E. coli. Cell 32:809–816

    Google Scholar 

  • Klaer R, Starlinger P (1980) IS4 at its chromosomal site in E. coli K12. Mol Gen Genet 178:285–291

    Google Scholar 

  • Klaer R, Kühn S, Tillmann E, Starlinger P (1981) The sequence of IS4. Mol Gen Genet 181:169–175

    Google Scholar 

  • Kohara Y, Akiyama K, Isono K (1987) The physical map of the whole E. coli chromosome: Application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50:495–508

    Google Scholar 

  • Louarn JM, Bouché JP, Legendre F, Louarn J, Patte J (1985) Characterization and properties of very large inversions of the E. coli chromosome along the origin-to-terminus axes. Mol Gen Genet 201:467–476

    Google Scholar 

  • Muramatsu S, Kato M, Kohara Y, Mizuno T (1988) Insertion sequence IS5 contains a sharply curved DNA structure at its terminus. Mol Gen Genet 214:433–438

    Google Scholar 

  • Ohtsubo H, Ohtsubo E (1978) Nucleotide sequence of an insertion sequence, IS1. Proc Natl Acad Sci USA 75:615–619

    Google Scholar 

  • Reif HJ, Saedler H (1975) IS1 is involved in deletion formation in the gal region of E. coli K12. Mol Gen Genet 137:17–28

    Google Scholar 

  • Reynolds AE, Felton J, Wright A (1981) Insertion of DNA activates the cryptic bgl operon in E. coli K12. Nature 293:625–629

    Google Scholar 

  • Saedler H, Heiß B (1973) Multiple copies of the insertion-DNA sequences IS1 and IS2 in the chromosome of E. coli K12. Mol Gen Genet 122:267–277

    Google Scholar 

  • Saedler H, Reif HJ, Hu S, Davidson N (1974) IS2, a genetic element for turn-off and turn-on of genetic activity in E. coli. Mol Gen Genet 132:265–289

    Google Scholar 

  • Saedler H, Kubai DF, Nomura M, Jaskunas SR (1975) IS1 and IS2 mutations in the ribosomal protein genes of E. coli K12. Mol Gen Genet 141:85–89

    Google Scholar 

  • Saedler H, Cornelis G, Cullum J, Schumacher B, Sommer H (1980) IS1-mediated DNA rearrangements. Cold Spring Harbour Symp Quant Biol 45:93–98

    Google Scholar 

  • Sawyer SA, Dykhuizen DE, DuBose RF, Green L, Mutangadura-Mhanga T, Wolczyk DF, Hartl DL (1987) Distribution and abundance of insertion sequences among natural isolates of Escherichia coli. Genetics 115:51–61

    Google Scholar 

  • Schwartz E, Kröger M, Rak B (1988) IS150: distribution, nucleotide sequence and phylogenetic relationships of a new E. coli insertion element. Nucleic Acids Res 16:6789–6808

    Google Scholar 

  • Starlinger P (1980) IS elements and transposons. Plasmid 3:241–259

    Google Scholar 

  • Timmerman KP, Tu C-P (1985) Complete sequence of IS3. Nucleic Acids Res 13:2127–2139

    Google Scholar 

  • Virolle MJ, Gèlugne JP, Bèjar S, Bouchè JP (1983) Origin of E. coli K12 Hfr B7. J Bacteriol 153:610–615

    Google Scholar 

  • Xia X-M, Enomoto M (1986) A naturally occurring large chromosomal inversion in E. coli K12. Mol Gen Genet 205:376–379

    Google Scholar 

  • Zerbib D, Gamas P, Chandler M, Prentki P, Bass S, Galas D (1985) Specificity of insertion of IS1. J Mol Biol 185:517–524

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institut für Genetik der Universität zu Köln, Weyertal 121, D-5000, Köln 41, Federal Republic of Germany

    Rainer P. Birkenbihl & Walter Vielmetter

Authors
  1. Rainer P. Birkenbihl
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Walter Vielmetter
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Communicated by H. Saedler

Rights and permissions

Reprints and permissions

About this article

Cite this article

Birkenbihl, R.P., Vielmetter, W. Complete maps of IS1, IS2, IS3, IS4, IS5, IS30 and IS150 locations in Escherichia coli K12. Molec. Gen. Genet. 220, 147–153 (1989). https://doi.org/10.1007/BF00260869

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00260869

Key words

Search

Navigation