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Pheromone-induced stimulation of inositol-trisphosphate formation in insect antennae is mediated by G-proteins

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Summary

Phospholipase C in the antennae of Periplaneta americana was stimulated by pheromones in a GTP-dependent manner. The enzyme activity was potentiated by hydrolysis-resistant analogs of GTP and decreased by GDP analogs. Guanine nucleotide binding regulatory proteins (G-proteins) in antennal preparations were identified by bacterial toxin-catalyzed ADP-ribosylation and immunoreactivity with antibodies of designed specificity. The stimulatory effect of pheromones on inositol phosphate formation was completely blocked by pertussis toxin suggesting that the pheromone action was mediated via specific G-proteins.

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Abbreviations

G-protein :

guanine nucleotide-binding regulatory protein

IP 3 :

inositol 1,4,5-trisphosphate

GTPS :

guanine-5′-[thio]-triphosphate

GDPβS :

guanine-5′-[β-thio]-diphosphate

NAD :

nicotinic acid adenine dinucleotide

SDS-PAGE :

sodium dodecylsulfate polyacrylamide gel electrophoresis

DTT :

dithiothreitol

MOPS :

3-(N-morpholino)propanesulfonic acid

PIPES :

piperazine-N,N′-bis[2-ethanesulfonic acid]

DMSO :

dimethyl-sulfoxide

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Authors and Affiliations

  1. Institut für Zoophysiologie, Universität Stuttgart-Hohenheim, Garbenstr. 30, D-7000, Stuttgart 70, Germany

    I. Boekhoff, K. Raming & H. Breer

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  1. I. Boekhoff
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  2. K. Raming
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  3. H. Breer
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Boekhoff, I., Raming, K. & Breer, H. Pheromone-induced stimulation of inositol-trisphosphate formation in insect antennae is mediated by G-proteins. J Comp Physiol B 160, 99–103 (1990). https://doi.org/10.1007/BF00258768

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  • DOI: https://doi.org/10.1007/BF00258768

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