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Einige Eigenschaften der RNS-Polymerase-Reaktion in Warmblüter-Mitochondrien

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Summary

Characteristics and kinetics of RNA-polymerase activity have been investigated in mitochondria isolated from pigeon heart and rat liver.

After incorporation of 3H- and 14C-labeled nucleotides in vitro the mitochondrial RNA was extracted using phenol or perchloric acid. Data are presented showing the time course of the reaction and the influence exerted by changing enzyme (mitochondrial protein) or substrate concentration. Accelerations of the incorporation rate were observed when pH-values, temperatur and Mg++ concentrations of the incubation mixture were increased.

A comparison of nuclear and mitochondrial RNA-polymerase activities reveals similarities of the basic incorporation process. However, distinct differences appear to be present as far as K m -values are concerned. Since the enzyme is characteristically located within the mitochondrial compartment, accessibility is limited by membrane impermeability. This provides additional proof for the conclusion that the incorporation process is a property of the mitochondria per se.

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Authors and Affiliations

  1. Pharmakologisches Institut der Freien Universität Berlin, Germany

    Diether Neubert, Hans Helge & Stephanie Teske

Authors
  1. Diether Neubert
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  2. Hans Helge
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  3. Stephanie Teske
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Universitäts-Kinderklinik, Freie Universität Berlin.

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Neubert, D., Helge, H. & Teske, S. Einige Eigenschaften der RNS-Polymerase-Reaktion in Warmblüter-Mitochondrien. Naunyn - Schmiedebergs Arch 252, 452–462 (1966). https://doi.org/10.1007/BF00258644

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  • DOI: https://doi.org/10.1007/BF00258644

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