Summary
The production of α-amylase and amyloglucosidase activity in the yeast Schwanniomyces castellii strain CBS 2863 is repressed in the presence of glucose. Mutants displaying increased amylase activity were obtained after treatment with UV light and screening for resistance to 2-deoxy-glucose. One mutant was found to exhibit derepressed amylase activity. Biosynthesis and the rate of excretion did not appear to be as highly sensitive to dissolved oxygen, pH and dilution rate as in the parental strain.
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Bergmeyer HV, Bernt E, Schmidt E, Stork H (1974) d-Glucose determination with hexokinase and glucose-6-phosphate dehydrogenase. In: Bergmeyer HV, Gawehnk A (eds) Methods of enzymatic analysis, vol 3. Academic Press, New York, 1196–1201
Boze H, Moulin G, Galzy P (1987a) Influence of culture conditions on the cell yield and amylases biosynthesis in continuous culture by Schwanniomyces castellii. Arch Microbiol 148:162–166
Boze H, Moulin G, Galzy P (1987b) A comparison of growth yields obtained from Schwanniomyces castellii and an alcohol dehydrogenase mutant. Biotechnol Lett 9:461–466
Boze H, Guyot JB, Moulin G, Galzy P (1989) Kinetics of the amyloglucosidase of Schwanniomyces castellii. Yeast 5:117–121
Bruinenberg PM, Dijken JP van, Scheffers WA (1983) An enzymic analysis of NADPH production and consumption in Candida utilis. J Gen Microbiol 129:965–971
De Mot R, Verachter H (1987) Regulation of the amylase secretion by Filobasidium capsuligenum and a 2-deoxy-d-glucose resistant mutant. Appl Microbiol Biotechnol 26:258–262
Dixon GH, Kornberg HB (1959) Assay methods for key enzymes of the glyoxylate cycle. Biochem J 72:3
Entian KD, Mecke D (1982) Genetic evidence for a role of hexokinase isoenzyme PII in carbon catabolite repression in Saccharomyces cerevisiae. J Biol Chem 257:870–874
Entian KD, Zimmermann FK, Scheel I (1977) A partial effect in carbon catabolite repression in mutants of Saccharomyces cerevisiae with reduced hexose phosphorylation. Mol Gen Genet 156:99–105
Galzy P (1964) Etude génétique et physiologique du métabolisme de l'acide lactique chez Saccharomyces cerevisiae Hansen. Ann Technol Agric (Paris) 13:109–259
Kreger van Rij NJN (1984) The yeast, a taxonomic study, 3rd edn. Elsevier, Amsterdam
MacCann AK, Barnett JA (1984) Starch utilisation by yeast: mutant resistant to carbon catabolite repression. Curr Genet 8:525–530
Maïtra PK (1970) A glucokinase from Saccharomyces cerevisiae. J Biol Chem 245:2423–2431
Oteng-Gyang K, Moulin G, Galzy P (1980) Effects of medium composition on excretion and biosynthesis of amylase of Schwanniomyces castellii. J Eur Appl Microbiol Biotechnol 9:129–132
Polakis ES, Bartley W (1965) Changes in the enzymes activities of Saccharomyces cerevisiae during aerobic growth on different carbon sources. Biochem J 97:284–297
Sills AM, Steward GG (1982) Production of amylolytic enzymes by several yeast species. J Inst Brew 88:313–316
Sills AM, Sauder ME, Steward GG (1984) Isolation and characterization of the amylolytic system of Schwanniomyces castellii. J Inst Brew 90:311–314
Smith BW, Roe JM (1957) A photometric method for the determination of α-amylase in blood and urine with use of the starch iodine color. J Biol Chem 7:53–59
Zimmermann FK, Scheel I (1977) Mutants of Saccharomyces cerevisiae resistant to carbon catabolite repression. Mol Gen Genet 154:75–82
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Boze, H., Guyot, J.B., Moulin, G. et al. Isolation and characterization of a derepressed mutant of Schwanniomyces castellii for amylase production. Appl Microbiol Biotechnol 31, 366–370 (1989). https://doi.org/10.1007/BF00257606
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DOI: https://doi.org/10.1007/BF00257606