Summary
Acetolactate decarboxylase activity has been detected among three genera, nine species and 263 strains of lactic acid bacteria tested in the course of a screening for acetolactate decarboxylases amenable for use in brewing as maturation aid. Streptococcus diacetylactis strain FD-64-D was found to generate a decarboxylase exhibiting a satisfactory activity and an excellent stability at the pH prevailing in beer and wort. This decarboxylase could not be solubilized but enzymatically active, freeze-dried cells were effective for satisfactory flavour maturation of beer although difficulties were encountered during attempts to remove the applied cell material by filtration of the beer. Lactobacillus casei DSM 2547 was likewise found to produce a decarboxylase exhibiting a satisfactory activity and stability at the low pH of beer and which, in addition, was readily solubilized. A method has been developed for pilot scale production of preparations of this decarboxylase suitable for use in brewing.
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Abbreviations
- DSM:
-
Deutsche Sammlung von Microorganismen
- EDTA:
-
Ethylene diaminetetra-acetic acid
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Godtfredsen, S.E., Rasmussen, A.M., Ottesen, M. et al. Occurrence of α-acetolactate decarboxylases among lactic acid bacteria and their utilization for maturation of beer. Appl Microbiol Biotechnol 20, 23–28 (1984). https://doi.org/10.1007/BF00254641
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DOI: https://doi.org/10.1007/BF00254641