Summary
Islets of Langerhans from NMRI-mice were kept for one week in tissue culture in medium supplemented with human serum obtained from either normal healthy subjects or newly diagnosed juvenile diabetic patients before insulin treatment. Islets cultured in diabetic serum released more inslin than islets cultured in normal serum, whether tissue culture medium 199 with 5.5–8.3 mmol/1 glucose and 10% serum, or culture medium RPMI1 640 with 11 mmol/1 glucose and 0.5% serum were used. Islets kept for one week in culture with diabetic serum did not show any decrease in DNA content or glucose induced insulin secretion and biosynthesis. It is concluded that serum from newly diagnosed insulin dependent diabetic patients stimulates insulin release from isolated mouse islets kept in tissue culture. The underlying mechanism is unknown.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Andersson A (1974) Long-term effects of glucose on insulin release and glucose oxidation in mouse pancreatic islets maintained in tissue culture. Biochem J 140: 377–382
Andersson A (1978) Isolated mouse pancreatic islets in culture: Effects of serum and different culture media on the insulin production of the islets. Diabetologia 14: 397–404
Andersson A, Hellerström C (1972) Metabolic characteristics of isolated pancreatic islets in tissue culture. Diabetes 21 [Suppl 2]: 546–554
Beloff-Chain A, Hawthorn J (1976) The release of insulin from pancreatic islets of lean and obese mice stimulated in vitro by pituitary glands from obese mice and by high glucose concentrations. FEES Lett 64: 214–217
Berne C (1975) Anti-insulin serum coupled to Sepharose 4 B as a tool for the investigation of insulin biosynthesis in the B- cells of obese hyperglycemie mice. Endocrinology 97: 1241–1247
Bottazzo GF, Florin-Christensen A, Doniach D (1974) Islet- cell antibodies in diabetes mellitus with autoimmune polyendocrine deficiencies. Lancet II: 1279–1283
Brunstedt J, Nielsen JH (1979) Direct long-term effect of hydrocortisone on insulin and glucagon release from pancreatic islets in tissue culture. Acta Endocrinol [Suppl] (Kbh) 227: 17
Buschard K, Andersen OO, Christau B, Christy M, Kromann H, Nerup J, Platz P, Svejgaard A, Thomsen M, Botazzo GF (1977) Islet cell antibodies — A marker of subclinical diabetes? A pathogenic factor? Diabetologia 13: 386
Eff C (1978) Insulin release, insulin content, and glucose utilization in isolated mouse islets maintained in tissue culture. Acta Endocrinol (Kbh) 87: 535–542
Green IC, Taylor KW (1972) Effects of pregnancy in the rat on the size and insulin secretory response of the islets of Langerhans. J Endocrinol 54: 317–325
Hahn HJ, Menzel R, Gottschling HD, Jahr D (1976) Enhancement of glucose-stimulated insulin secretion from isolated rat pancreatic islets by human insulin antibodies. Acta Endocrinol (Kbh) 83: 123–132
Hales CN, Randle PJ (1963) Immunoassay of insulin with insulin antibody precipitate. Biochem J 88: 137–146
Hanssen KF (1974) Immunoreactive growth hormone in plasma and urine in juvenile diabetics before and during initial insulin treatment. Acta Endocrinol (Kbh) 75: 50–63
Heding L (1972) Determination of total serum insulin (IRI) in insulin-treated diabetic patients. Diabetologia 8: 260–266
Hill DE, Mayes S, DiBattista D, Lockhart-Ewart R, Martin JM (1977) Hypothalamic regulation of insulin release in rhesus monkeys. Diabetes 26: 726–731
Johnson LR (1977) Gastrointestinal hormones and their functions. Annu Rev Physiol 39: 135–158
Lautala P, Åkerblom HK, Kouvalainen K, Martin JM (1977) A hypothalamic islet-stimulating factor and childhood obesity. In: Chiumello G, Laron Z (eds) Recent progress in pediatric endocrinology. Serono Symposia No 12. Academic Press, London, p 235–239
Lazarow A, Wells LJ, Carpenter AM, Hegre OD, Leonard RJ, McEvoy RC (1973) Islet differentiation, organ culture, and transplantation. Diabetes 22: 877–912
Lernmark Å, Hägglöf B, Holmgren G, Ludvigsson J (1979) Antibodies against the pancreatic B-cell plasma membrane in insulin-dependent diabetic children. Acta Endocrinol [Suppl] (Kbh) 227: 48–49
Lernmark Å, Sehlin J, Täljedal I-B, Kromann H, Nerup J (1978) Possible toxic effects of normal and diabetic patient serum on pancreatic B-cells. Diabetologia 14: 25–31
Moltz JH, Dobbs RE, McCann SM, Fawcett CP (1977) Effects of hypothalamic factors on insulin and glucagon release from islets of Langerhans. Endocrinology 101: 196–202
Nielsen E, Asfeldt VH (1967) Studies on the specificity of fluorometric determination of plasma corticosteroids ad modum DeMoor & Steeno. Scand J Clin Lab Invest 20: 185–194
Nielsen JH, Brunstedt J, Andersson A, Frimodt-Møller C (1979) Preservation of beta cell function in adult human pancreatic islets for several months in vitro, Diabetologia 16: 97–100
Nielsen JH, Brunstedt K, Jørgensen R, Jensen D (1978) Direct long-term effect of growth hormone and prolactin on growth and function of pancreatic islets in tissue culture. Diabetologia 15: 258
Weerasinghe C, Bornstein J (1978) Effect of synthetic C-terminal fragments of HGH on insulin release by isolated islets. Am J Physiol 234: E527-E531
Zimmet P, Ng FM, Bornstein J, Armstrong J McD, Taft HP (1971) Insulin antagonist of pituitary origin in plasma of normal and diabetic subjects. Br Med J I: 203–204
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Nielsen, J.H., Eff, C., Deckert, T. et al. Stimulatory effect of serum from diabetic patients on insulin release from mouse pancreatic islets maintained in tissue culture. Diabetologia 20, 60–65 (1981). https://doi.org/10.1007/BF00253819
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00253819