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Isolation, Purification, and Culture of Mouse Pancreatic Islets of Langerhans

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Mouse Cell Culture

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1940))

Abstract

Pancreatic islets constitute an important tool for research and clinical applications in the field of diabetes. They are used for transplantation, unraveling new mechanisms in insulin secretion, studying pathophysiological pathways in diseased cells, and pharmacological research aimed at developing improved therapeutic strategies. Therefore, fine-tuning islet isolation protocols remains an important objective for reliable investigations. Here we describe a relatively simple mouse islet isolation protocol that relies on enzymatic digestion using low-activity collagenase and several sedimentation and Percoll gradient steps.

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Authors and Affiliations

  1. Laboratoire de Recherche en Physiologie et Physiopathologie LRPP, Pôle Technologie Santé, Faculté de Médecine, Université Saint Joseph, Beirut, Lebanon

    Youakim Saliba & Nassim Farès

Authors
  1. Youakim Saliba
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  2. Nassim Farès
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Corresponding author

Correspondence to Nassim Farès .

Editor information

Editors and Affiliations

  1. Lung Health Research Centre, Department of Pharmacology and Therapeutics, University of Melbourne, Melbourne, VIC, Australia

    Ivan Bertoncello

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Saliba, Y., Farès, N. (2019). Isolation, Purification, and Culture of Mouse Pancreatic Islets of Langerhans. In: Bertoncello, I. (eds) Mouse Cell Culture. Methods in Molecular Biology, vol 1940. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9086-3_18

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  • DOI: https://doi.org/10.1007/978-1-4939-9086-3_18

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-9085-6

  • Online ISBN: 978-1-4939-9086-3

  • eBook Packages: Springer Protocols

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