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Enhanced Inulin Saccharification by Self-Produced Inulinase from a Newly Isolated Penicillium sp. and its Application in d-Lactic Acid Production

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Abstract

In order to find an alternative for commercial inulinase, a strain XL01 identified as Penicillium sp. was screened for inulinase production. The broth after cultivated was centrifuged, filtered, and used as crude enzyme for the following saccharification. At pH 5.0 and 50 °C, the crude enzyme released 84.9 g/L fructose and 20.7 g/L glucose from 120 g/L inulin in 72 h. In addition, simultaneous saccharification and fermentation of chicory flour for d-lactic acid production was carried out using the self-produced crude inulinase and Lactobacillus bulgaricus CGMCC 1.6970. A high d-lactic acid titer and productivity of 122.0 g/L and 1.69 g/(L h) was achieved from 120 g/L chicory flour in 72 h. The simplicity for inulinase production and the high efficiency for d-lactic acid fermentation provide a perspective and profitable industrial biotechnology for utilization of the inulin-rich biomass.

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Acknowledgments

This study was supported by the National Natural Science Foundation of China (51776099, 31300487), the Key Research and Development Program of Jiangsu Province of China (BF2015007). The authors are also grateful to the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD).

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Author notes

  1. Zhaojuan Zheng and Qianqian Xu contributed equally to this work.

Authors and Affiliations

  1. Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, Nanjing Forestry University, Nanjing, 210037, People’s Republic of China

    Zhaojuan Zheng, Qianqian Xu, Fan Zhou & Jia Ouyang

  2. College of Chemical Engineering, Nanjing Forestry University, Nanjing, 210037, People’s Republic of China

    Zhaojuan Zheng & Jia Ouyang

  3. College of Forestry, Nanjing Forestry University, Nanjing, 210037, People’s Republic of China

    Peng Liu

  4. Key Laboratory of Forest Genetics and Biotechnology of the Ministry of Education, Nanjing, 210037, People’s Republic of China

    Jia Ouyang

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  1. Zhaojuan Zheng
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  2. Qianqian Xu
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  3. Peng Liu
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  4. Fan Zhou
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Correspondence to Jia Ouyang.

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Zheng, Z., Xu, Q., Liu, P. et al. Enhanced Inulin Saccharification by Self-Produced Inulinase from a Newly Isolated Penicillium sp. and its Application in d-Lactic Acid Production. Appl Biochem Biotechnol 186, 122–131 (2018). https://doi.org/10.1007/s12010-018-2730-6

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  • DOI: https://doi.org/10.1007/s12010-018-2730-6

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