Abstract
Cell extracts of Archaeoglobus fulgidus were found to catalyze an isotope exchange between CO2 and the carbonyl group of acetyl-CoA. This observation and the presence of carbon monoxide: methyl viologen oxidoreductase activity strongly support the recent proposal that in A. fulgidus acetyl-CoA is degraded via a decarbonylation reaction.
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References
Achenbach-Richter L, Stetter KO, Woese CR (1987) A possible biochemical missing link among archaebacteria. Nature (London) 327: 348–349
Fischer R, Thauer RK (1989a) Methyltetrahydromethanopterin as an intermediate in methanogenesis from acetate in Methanosarcina barkeri. Arch Microbiol 151: 459–465
Fischer R, Thauer RK (1989b) Methanogenesis from acetate in cell extracts of Methanosarcina barkeri: Isotope exchange between CO2 and the carbonyl group of acetyl-CoA, and the role of H2. Arch Microbiol (in press)
Fuchs G (1986) CO2 fixation in acetogenic bacteria: variations on a theme. FEMS Microbiol Rev 39: 181–213
Harder SR, Lu WP, Feinberg BA, Ragsdale SW (1989) Spectro-electrochemical studies of the corrinoid/iron-sulfur protein involved in acetyl-CoA synthesis by Clostridium thermoaceticum. Biochemistry (in press)
Jansen K, Fuchs G, Thauer RK (1985) Autotrophic CO2 fixation by Desulfovibrio baarsii: demonstration of enzyme activities characteristic for the acetyl-CoA pathway: FEMS Microbiol Lett 28: 311–315
Länge S, Fuchs G (1987) Autotrophic synthesis of activated acetic acid from CO2 in Methanobacterium thermoautotrophicum. Synthesis from tetrahydromethanopterin-bound C1 units and carbon monoxide. Eur J Biochem 163: 147–154
Länge S, Scholtz R, Fuchs G (1989) Oxidative and reductive acetyl CoA/carbon monoxide dehydrogenase pathway in Desulfobacterium autotrophicum. Arch Microbiol 151: 77–83
Möller-Zinkhan D, Thauer RK (1988) Membrane-bound NADPH dehydrogenase- and ferredoxin: NADP oxidoreductase activity involved in electron transport during acetate oxidation to CO2 in Desulfobacter postgatei. Arch Microbiol 150: 145–154
Möller-Zinkhan D, Börner G, Thauer RK (1989) Function of methanofuran, tetrahydromethanopterin, and coenzyme F420 in Archaeoglobus fulgidus. Arch Microbiol 152: 362–368
Pezacka E, Wood HG (1988) Acetyl-CoA pathway of autotrophic growth. Identification of the methyl-binding site of the CO dehydrogenase. J Biol Chem 263: 16000–16006
Ragsdale SW, Wood HG (1985) Acetate biosynthesis by acetogenic bacteria. Evidence that carbon monoxide dehydrogenase is the condensing enyzme that catalyzes the final steps of the synthesis. J Biol Chem 260: 3970–3977
Ramer SE, Raybuck SA, Orme-Johnson WH, Walsh CT (1989) Kinetic characterization of the [3′-32P]coenzyme A/acetyl coenzyme A exchange catalyzed by a three-subunit form of the carbon monoxide dehydrogenase/acetyl-CoA synthase from Clostridium thermoaceticum. Biochemistry 28: 4675–4680
Raybuck SA, Bastian NR, Orme-Johnson WH, Walsh CT (1988) Kinetic characterization of the carbon monoxide-acetyl-CoA (carbonyl group) exchange activity of the acetyl-CoA synthesizing CO dehydrogenase from Clostridium thermoaceticum. Biochemistry 27: 7698–7702
Roberts DL, James-Hagstrom JE, Garvin DK, Gorst CM (1989) Cloning and expression of the gene cluster encoding key proteins involved in acetyl-CoA synthesis in Clostridium thermoaceticum: CO dehydrogenase, the corrinoid/Fe−S protein, and methyltransferase. Proc Natl Acad Sci USA 86: 32–36
Russ E (1987) Physiologisch-biochemische Untersuchungen an dem extrem thermophilen archaebakteriellen Sulfatreduzierer Archaeoglobus fulgidus. Diploma thesis, University of Regensburg
Schauder R, Eikmanns B, Thauer RK, Widdel F, Fuchs G (1986) Acetate oxidation to CO2 in anaerobic bacteria via a novel pathway not involving reactions of the citric acid cycle. Arch Microbiol 145: 162–172
Schauder R, Preuß A, Jetten M, Fuchs G (1989) Oxidative and reductive acetyl CoA/carbon monoxide dehydrogenase pathway in Desulfobacterium autotrophicum. 2. Demonstration of the enzymes of the pathway and comparison of CO dehydrogenase. Arch Microbiol 151: 84–89
Shanmugasundaram T, Kumar GK, Wood HG (1988) Involvement of tryptophan residues at the coenzyme A binding site of carbon monoxide dehydrogenase from Clostridium thermoaceticum. Biochemistry 27: 6499–6503
Speich N, Trüper HG (1988) Adenylylsulphate reductase in a dissimilatory sulphate-reducing archaebacterium. J Gen Microbiol 134: 1419–1425
Spormann AM, Thauer RK (1988) Anaerobic acetate oxidation to CO2 by Desulfotomaculum acetoxidans. Demonstration of enzymes required for the operation of an oxidative acetyl-CoA/carbon monoxide dehydrogenase pathway. Arch Microbiol 150: 374–380
Spormann AM, Thauer RK (1989) Anaerobic acetate oxidation to CO2 by Desulfotomaculum acetoxidans. Isotopic exchange between CO2 and the carbonyl group of acetyl-CoA and topology of enzymes involved. Arch Microbiol 152: 189–195
Stetter KO (1988) Archaeoglobus fulgidus gen. nov., sp. nov.: a new taxon of extremely thermophilic archaebacteria. System Appl Microbiol 10: 172–173
Stetter KO, Lauerer G, Thomm M, Neuner A (1987) Isolation of extremely thermophilic sulfate reducers: Evidence for a novel branch of archaebacteria. Science 236: 822–824
Thauer RK (1988) Citric-acid cycle, 50 years on. Modifications and an alternative pathway in anaerobic bacteria. Eur J Biochem 176: 497–508
Thauer RK (1989) Energy metabolism of sulfate-reducing bacteria. In: Schlegel HG, Bowien B (eds) Autotrophic bacteria. Science Tech Publishers. Madison, WI, Springer, Berlin Heidelberg New York London Paris Tokyo, pp 397–413
Thauer RK, Möller-Zinkhan D, Spormann AM (1989) Biochemistry of acetate catabolism in anaerobic chemotrophic bacteria. Annu Rev Microbiol 43: 43–67
Vogel HU von (1974) Chemiker-Kalender, Springer Berlin Heidelberg New York, p 486
Widdel F (1988) Microbiology and ecology of sulfate- and sulfur-reducing bacteria. In: Zehnder AJB (ed) Biology of anaerobic microorganisms. John Wiley & Sons, New York Chichester Brisbane Toronto Singapore, pp 469–585
Wood HG, Ragsdale SW, Pezacka E (1986) The acetyl-CoA pathway of autotrophic growth. FEMS Microbiol Rev 39: 345–362
Zellner G, Stackebrandt E, Kneifel H, Messner P, Sleytr UB, Conway de Macario E, Zabel HP, Stetter KO (1989) Isolation and characterization of a thermophilic, sulfate reducing archaebacterium, Archaeoglobus fulgidus strain Z. Syst Appl Microbiol 11: 151–160
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Möller-Zinkhan, D., Thauer, R.K. Anaerobic lactate oxidation to 3 CO2 by Archaeoglobus fulgidus via the carbon monoxide dehydrogenase pathway: demonstration of the acetyl-CoA carbon-carbon cleavage reaction in cell extracts. Arch. Microbiol. 153, 215–218 (1990). https://doi.org/10.1007/BF00249070
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DOI: https://doi.org/10.1007/BF00249070