Abstract
Dibenzothiophene, a polycyclic aromatic sulfur heterocycle, represents as a model compound the organic sulfur integrated in the macromolecular coal matrix. A pure culture of a Brevibacterium species was isolated, which is able to use dibenzothiophene as sole source of carbon, sulfur and energy for growth. During dibenzothiophene utilization sulfite was released in a stoichiometrical amount and was further oxidized to sulfate. Three metabolites of dibenzothiophene degradation were isolated and identified as dibenzothiophene-5-oxide, dibenzothiophene-5-dioxide and benzoate by cochromatography, UV spectroscopy and gas chromatographymass spectrometry analyses. Based on the identified metabolites a pathway for the degradation of dibenzothiophene by Brevibacterium sp. DO is proposed.
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Abbreviations
- DBT:
-
dibenzothiophene
- PASH:
-
polycyclic aromatic sulfur heterocycle
- PAH:
-
polycyclic aromatic hydrocarbons
- GC-MS:
-
gas chromatography-mass spectrometry
- HPLC:
-
high pressure liquid chromatography
- IC:
-
ion chromatography
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van Afferden, M., Schacht, S., Klein, J. et al. Degradation of dibenzothiophene by Brevibacterium sp.DO. Arch. Microbiol. 153, 324–328 (1990). https://doi.org/10.1007/BF00249000
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DOI: https://doi.org/10.1007/BF00249000