Abstract
(1) The electron transport system of heterotrophically dark-grown Rhodobacter capsulatus was investigated using the wild-type strain MT1131 and the phototrophic non-competent (Ps-) mutant MT-GS18 carrying deletions of the genes for cytochrome c 1 and b of the bc 1 complex and for cytochrome c 2. (2) Spectroscopic and thermodynamic data demonstrate that deletion of both bc 1 complex and cyt. c 2 still leaves several haems of c- and b-type with Em7.0 of +265 mV and +354 mV at 551–542 nm, and +415 mV and +275 mV at 561–575 nm, respectively. (3) Analysis of the oxidoreduction kinetic patterns of cytochromes indicated that cyt. b 415 and cyt. b 275 are reduced by either ascorbate-diaminodurene or NADH, respectively. (4) Growth on different carbon and nitrogen sources revealed that the membrane-bound electron transport chain of both MT1131 and MT-GS18 strains undergoes functional modifications in response to the composition of the growth medium used. (5) Excitation of membrane fragments from cells grown in malate minimal medium by a train of single turnover flashes of light led to a rapid oxidation of 32% of the membrane-bound c-type haem complement. Conversely, membranes prepared from peptone/yeast extract grown cells did not show cyt. c photooxidation. These results are discussed within the framework of an electron transport chain in which alternative pathways bypassing both the cyt. c 2 and bc 1 complex might involve high-potential membrane bound haems of b- and c-type.
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Abbreviations
- AA:
-
antimycin A
- CCCP:
-
carbonylcyanide m-chlorophenyl hydrazone
- CN- :
-
cyanide
- DAD:
-
diaminodurene
- Q2H2 :
-
ubiquinol-2
- Q-pool:
-
ubiquinone-10 pool
- RC:
-
photochemical reaction center
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Zannoni, D., Venturoli, G. & Daldal, F. The role of the membrane bound cytochromes of b- and c-type in the electron transport chain of Rhodobacter capsulatus . Arch. Microbiol. 157, 367–374 (1992). https://doi.org/10.1007/BF00248683
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DOI: https://doi.org/10.1007/BF00248683