Summary
Corynebacterium glutamicum effectively secretes isoleucine when the precursor 2-ketobutyrate is added to the medium. Isoleucine secretion was studied under different conditions with respect to various parameters, i.e. rate of isoleucine excretion and uptake, concentration gradients of isoleucine, other amino acids and ions, and membrane potential. By comparing these parameters in the presence and absence of the amino acid precursor it has been shown that the efflux of isoleucine in C. glutamicum can neither be explained by a passive diffusion mechanism nor by a process involving functional inversion of the isoleucine uptake carrier. Based on our results concerning the distribution of metabolites and the kinetics of excretion we conclude that isoleucine is excreted in C. glutamicum by a separate, presumably active efflux carrier system.
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Ebbighausen, H., Weil, B. & Krämer, R. Isoleucine excretion in Corynebacterium glutamicum: evidence for a specific efflux carrier system. Appl Microbiol Biotechnol 31, 184–190 (1989). https://doi.org/10.1007/BF00262460
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DOI: https://doi.org/10.1007/BF00262460