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Copper affinity precipitation as an initial step in protein purification

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Biotechnology Techniques

Summary

The affinity of copper ions for histidine residues can be utilized to carry out fractional precipitation of proteins. Using this approach it is possible to purify concanavalin A by a simple two step procedure. The fold purification obtained was 4.5 fold.

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Authors and Affiliations

  1. Department of Chemistry, Indian Institute of Technology, 110 016, Delhi, India

    Ritu Agarwal & M. N. Gupta

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  1. Ritu Agarwal
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  2. M. N. Gupta
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Agarwal, R., Gupta, M.N. Copper affinity precipitation as an initial step in protein purification. Biotechnol Tech 8, 655–658 (1994). https://doi.org/10.1007/BF00241693

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  • DOI: https://doi.org/10.1007/BF00241693

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