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Plant regeneration from callus cultures of Piper longum L. by organogenesis

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Abstract

Plant regeneration from callus cultures of Piper longum was achieved through organogenesis. In vitro grown shoots were used as explants for callus induction. Competent callus was initiated around the nodal ring of tissue using Murashige and Skoog medium supplemented with 1.0 mg.l−1α- naphthaleneacetic acid and 0.2 mg.l−1 N6-benzyladenine. Optimum growth regulator concentrations for shoot induction and shoot elongation were found to be 0.5 mg.l−1 indole-3-acetic acid with 1.5 mg.l−1 benzyladenine, and 0.1 mg.l−1 indole-3-acetic acid with 0.2 mg.l−1 benzyladenine, respectively. Elongated shoots were rooted on half-strength Murashige and Skoog medium having 0.1 mg.l−1 indole3-acetic acid. The rooted plants were successfully established in soil.

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Abbreviations

BA, N6 :

Benzyladenine

2, 4-D:

2, 4- dichlorophenoxyacetic acid

IAA:

Indole-3-acetic acid

2iP:

2-isopentenyladenine

Kn:

Kinetin

MS:

Murashige and Skoog (1962)

NAA:

α-Naphthaleneacetic acid

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Authors and Affiliations

  1. National Plant Tissue Culture Repository, NBPGR, Pusa Campus, 110012, New Delhi, India

    S. R. Bhat, A. Kackar & K. P. S. Chandel

Authors
  1. S. R. Bhat
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  2. A. Kackar
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  3. K. P. S. Chandel
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Additional information

Communicated by G. C. Phillips

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Bhat, S.R., Kackar, A. & Chandel, K.P.S. Plant regeneration from callus cultures of Piper longum L. by organogenesis. Plant Cell Reports 11, 525–528 (1992). https://doi.org/10.1007/BF00236270

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  • DOI: https://doi.org/10.1007/BF00236270

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