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Amino acid transport in the rat exocrine pancreas

III. Effect of maximal and supramaximal hormonal stimulation in vivo

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Summary

Amino acid transport and incorporation have been studied in vitro in rat pancreatic lobules after maximal and supramaximal hormonal stimulation with caerulein. Incorporation into proteins was increased already after 30 and 120 min of maximal stimulation, but was decreased after the infusion of a supramaximal dose. Uptake of neutral amino acids was monitored using labeled leucine and α-aminoisobutyric acid (AIB). In the case of leucine the free pool was consistently reduced after maximal stimulation, while supramaximal doses led to an increase which could be potentiated by the addition of 2mM tetracaine. Using AIB, a significant increase in the intracellular pool was observed after maximal stimulation, conversely a decrease after supramaximal stimulation. Release of labeled leucine and AIB from preloaded lobules during incubation in the cold was significantly reduced after maximal secretory stimulation, but was found enhanced by 200 to 300 percent after supramaximal stimulation. No fine structural alterations at junctional complexes or at both the lateral and luminal plasma membranes were observed after maximal stimulation except an increased number of exocytotic figures at the luminal face. However, supramaximal stimulation led to progressive rarefaction of the tight junctional network and disintegration of the gap junctions. Concomitantly, an equal distribution of membrane particles on both faces of the plasma membrane together with a random occurrence of exocytotic figures were observed.

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References

  • Adler, G., Kern, H.F.: Membrane alterations after maximal and supramaximal secretory stimulation. A freeze-etch study. (In preparation)

  • Bieger, W., Martin-Achard, A., Bassler, M., Kern, H.F.: Studies on intracellular transport of secretory proteins in the rat exocrine pancreas. IV. Stimulation by in vivo infusion of caerulein. Cell Tiss. Res. 165, 435–453 (1976)

    Google Scholar 

  • Bieger, W., Peter, S., Kern, H.F.: Amino acid transport in the rat exocrine pancreas. I. Transport of neutral amino acids and their utilization in protein synthesis. Cell Tiss. Res. 180, 31–44 (1977a)

    Google Scholar 

  • Bieger, W., Peter, S., Völkl, A., Kern, H.F.: Amino acid transport in the rat exocrine pancreas. II. Inhibition by lanthanum and tetracaine. Cell Tiss. Res. 180, 45–62 (1977b)

    Google Scholar 

  • Bieger, W., Weicker, H., Haymovits, A.: Amino acid transport in the rat exocrine pancreas. IV. Insulin and glucagon mediate the in vivo effect of caerulein on amino acid transport and incorporation. (In press)

  • Case, M.: Synthesis, intracellular transport and discharge of exportable proteins in the pancreatic acinar cell and other cells. Biol. Rev. 53, 211–354 (1978)

    Google Scholar 

  • Cheneval, J.P., Johnstone, R.M.: Changes in amino acid transport in the rat pancreas in response to fasting and feeding. Biochim. biophys. Acta (Amst.) 433, 630–637 (1976)

    Google Scholar 

  • Christensen, H.N., De Cespedes, C., Handlongten, M.E., Ronquist, G.: Energization of amino acid transport, studies for the Ehrlich ascites tumor cell. Biochim. biophys. Acta (Amst.) 300, 487–522 (1973)

    Google Scholar 

  • Clemens, R.J., Pain, V.M.: Hormonal requirements for acute stimulation of rat liver polysome formation by amino acid feeding. Biochim. biophys. Acta (Amst.) 361, 345–357 (1974)

    Google Scholar 

  • Cooper, G.J., Kohn, P.G.: The effect of tetracaine on 2-amino-isobutyric acid transport in muscle. J. Physiol. (Lond.) 229, 23–24 (1973)

    Google Scholar 

  • Danielsson, A., Sehlin, J.: Transport and oxidation of amino acids and glucose in the isolated exocrine mouse pancreas: effects of glucose, insulin and pancreozymin. Acta physiol. scand. 91, 557–565 (1974)

    Google Scholar 

  • Decker, R.S.: Hormonal regulation of gap junction differentiation. J. Cell Biol. 69, 669–685 (1976)

    Google Scholar 

  • Dermietzel, R., Meller, K., Tetzlaff, W., Waelsch, M.: In vivo and in vitro formation of the junctional complex in choroid epithelium. A freeze-etching study. Cell Tiss. Res. 181, 427–441 (1977)

    Google Scholar 

  • Eagle, M.: Amino acid metabolism in mammalian cell structures. Science 130, 245–247 (1959)

    Google Scholar 

  • Galli, P., Brenna, A., De Camilli, P., Meldolesi, J.: Extracellular calcium and the organization of tight junctions in pancreatic acinar cells. Exp. Cell Res. 99, 179–183 (1976)

    Google Scholar 

  • Humbert, F., Montesano, R., Perrelet, A., Orci, L.: Junctions in developing human and rat kidney: a freeze-fracture study. J. Ultrastruct. Res. 56, 202–214 (1976)

    Google Scholar 

  • Ito, S., Karnovsky, M.J.: Formaldehyde-glutaraldehyde fixatives containing trinitro compounds. J. Cell Biol. 39, 168a (1968)

  • Kletzien, R.F., Pariza, M.W., Becker, J.E., Potter, V.R., Butcher, F.R.: Induction of amino acid transport in primary cultures of adult rat liver parenchymal cells by insulin. J. biol. Chem. 251, 3014–3020 (1976)

    Google Scholar 

  • Lampel, M., Kern, H.F.: Acute interstitial pancreatitis in the rat induced by excessive doses of a pancreatic secretagogue. Virchows Arch. Abt.A 373, 97–117 (1977)

    Google Scholar 

  • Metz, J., Merlo, M., Billich, H., Forssmann, W.G.: Exocrine pancreas under experimental conditions. IV. Alterations of intercellular junctions between acinar cells following pancreatic duct ligation. Cell Tiss. Res. 186, 227–240 (1978)

    Google Scholar 

  • Montesano, R., Friend, D.S., Perrelet, A., Orci, L.: In vivo assembly of tight junctions in fetal rat liver. J. Cell. Biol. 67, 310–319 (1975)

    Google Scholar 

  • Morisset, J.A., Webster, P.D.: Effects of fasting and feeding on protein synthesis by the rat pancreas. J. clin. Invest. 51, 1–8 (1972)

    Google Scholar 

  • Pannese, E., Luciano, L., Jurato, S., Reale, E.: Intercellular junctions and other membrane specializations in developing spinal ganglia: a freeze-fracture study. J. Ultrastruct. Res. 60, 169–180 (1977)

    Google Scholar 

  • Reggio, H., Cailla-Deckmayn, H., Marchis-Mouren, G.: Effect of pancreozymin on rat pancreatic enzyme biosynthesis. J. Cell Biol. 50, 333–343 (1971)

    Google Scholar 

  • Richards, G.M.: Modifications of the diphenylamine reaction giving increased sensitivity and simplicity in the estimation of DNA. Anal. Biochem. 57, 369–376 (1974)

    Google Scholar 

  • Robberecht, P., Deschodt-Lanckman, M., Canns, J., Kutzner, R., Cristophe, F.: Amino acid levels in rat pancreas after pilocarpine or pancreozymine. Amer. J. Physiol. 224, 1309–1313 (1973)

    Google Scholar 

  • Scheele, G.A., Palade, G.E.: Studies on the guinea pig pancreas. Parallel discharge of exocrine enzyme activities. J. biol. Chem. 250, 2660–2670 (1975)

    Google Scholar 

  • Staehelin, L.A.: Structure and function of intercellular junctions. Int. Rev. Cytol. 39, 191–283 (1974)

    Google Scholar 

  • Staehelin, L.A., Hull, B.E.: Junctions between living cells. Scient. Amer. 238, 141–153 (1978)

    Google Scholar 

  • Wanson, J.C., Drochmans, P., Mosselmans, R., Rouveaux, M.F.: Adult rat hepatocytes in primary monolayer culture. Ultrastructural characteristics of intercellular contacts and cell membrane differentiations. J. Cell Biol. 74, 858–877 (1977)

    Google Scholar 

  • Webster, P.D., Black, O., Mainz, D.L., Singh, M.: Pancreatic acinar cell metabolism and function. Gastroenterol. 73, 1434–1449 (1977)

    Google Scholar 

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Authors and Affiliations

  1. Department of Cell Biology and Cell Pathology, Philipps University, Marburg, Federal Republic of Germany

    Guido Adler, Wilfried Bieger &  Horst F. Kern

Authors
  1. Guido Adler
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  2. Wilfried Bieger
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  3. Horst F. Kern
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Additional information

Supported by a grant from the Deutsche Forschungsgemeinschaft, Bonn-Bad Godesberg (SFB 122, project C 5). Dedicated to Professor Dr. Gerhard Petry, Marburg, on the occasion of his 65th birthday

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Adler, G., Bieger, W. & Kern, H.F. Amino acid transport in the rat exocrine pancreas. Cell Tissue Res. 194, 447–462 (1978). https://doi.org/10.1007/BF00236165

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