Abstract
Two cell lines of Tabernaemontana divaricata derived from the same suspension culture accumulate different amounts of the terpenoid indole alkaloids O-acetylvallesamine and voaphylline. [15N]O-acetylvallesamine and [15N]voaphylline were added to the suspension cultures to investigate whether the lack of accumulating capacity of one of the cell lines was due to a low biosynthetic ability or to high turnover rates. The difference was shown to be due to the inability of the cell culture to biosynthesize both alkaloids. Both cell lines were able to metabolize O-acetylvallesamine. This metabolisation occurred mainly during the stationary phase. The alkaloids added were chemically unstable under culture conditions. Under normal batch cell culture conditions chemical breakdown is thought to play a minor role in the total amount of compound transformed.
Similar content being viewed by others
Abbreviations
- GC-MS:
-
gas chromatography-mass spectrometry
- HPLC-UV:
-
high performance liquid chromatography-ultra violet
- rpm:
-
rotations per minute
References
Dagnino D, Schripsema J, Peltenburg-Looman A, Verpoorte R, Teunis K (1991) J Nat Prod 54:1558–1563
Dagnino D, Schripsema J, Verpoorte R (1993) Phytochemistry 32:325–329
dos Santos RI, Schripsema J, Verpoorte R Phytochemistry, in press (1993)
Ghini AA, Burton G, Gros EG (1991) Phytochemistry 30:779–784
Goodbody A, Endo T, Vukovic J, Misawa M (1988) Planta Medica 210–214
Luckner M (1990) Secondary Metabolism in Microorganisms Plants and Animals. 3rd edn, Springer, Berlin Heidelberg New York
Murashige T, Skoog F (1962) Physiol Plant 15:473–497
Sariaslani FS, Duffel MW Rosazza JP (1985) J Med Chem 28:629–633
Schripsema J, Meijer AH, Iren F van, Hoopen HJG ten, Verpoorte R (1990) Plant Cell Tissue Org Cult 8:153–161
Schripsema J, Verpoorte R (1992) Planta Med 58:245–249
Sierra MI (1991) Ph D Thesis, University of Leiden, The Netherlands, pp 67–75
Sundberg RJ (1970) The Chemistry of Indoles. Academic Press, London
Author information
Authors and Affiliations
Additional information
Communicated by W. Barz
Rights and permissions
About this article
Cite this article
Dagnino, D., Schripsema, J. & Verpoorte, R. Comparison of terpenoid indole alkaloid production and degradation in two cell lines of Tabernaemontana divaricata . Plant Cell Reports 13, 95–98 (1993). https://doi.org/10.1007/BF00235298
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00235298