Skip to main content
SpringerLink
Log in

Autoradiographic detection of indolamine and catecholamine neurons in the nervous system of Owenia fusiformis (Polychaeta, Annelida)

  • Published:
Cell and Tissue Research Aims and scope Submit manuscript

Summary

Light- and electron-microscopic autoradiographic studies were carried out on Owenia to detect selectively catecholaminergic and indolaminergic neurons at two appropriate body levels according to the regenerative properties of this annelid, i.e. in the 1st and the 4th abdominal segments. Autoradiographically, intense 3H-5-hydroxytryptamine accumulation is seen in the 1st abdominal segment, and a less intense autoradiographic reaction in the 4th segment. Roughly, a similar difference in the distribution of the label is seen at both levels following 3H-noradrenaline administration. These observations, confirmed by a quantitative uptake analysis, suggest the presence of catecholaminergic and indolaminergic neurons in Owenia.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Aghajanian, G.K., Bloom, F.E., Lowell, R.A., Sheard, M., Freedman, D.X.: The uptake of 5-hydroxytryptamine-3H from the cerebral ventricles: autoradiographic localization. Biochem. Pharmacol. 15, 1401–1403 (1966)

    Google Scholar 

  • Bessone, R., Segu, L., Calas, A.: Radioautographic identification of serotoninergic neurons in Aplysia. Experentia 34, 1038–1039 (1978)

    Google Scholar 

  • Chapron, C., Chapron, J.: Influence des amines biogènes et de leurs inhibiteurs sur la régénération. Etude chez l'Annélide Eisenia foetida. C.R. Acad. Sci., Paris 274, D, 412–414 (1972)

    Google Scholar 

  • Clarck, M.E.: Histochemical localization of monoamines in the nervous system of the polychaete Neptys. Proc. Roy. Soc. B. 165, 308–325 (1966)

    Google Scholar 

  • Coggeshall, R.E.: Autoradiographic and chemical localization of 5-hydroxytryptamine in identified neurons in the leech. Anat. Rec. 172, 489–498 (1972)

    Google Scholar 

  • Coulon, J., Marilley, M.: Fluctuations of adenylate cyclase activity during anterior regeneration in Owenia fusiformis (Polychaete Annelid). J. Embryol. Exp. Morph., 48, 73–78 (1978)

    Google Scholar 

  • Coulon, J., Thouveny, Y.: Etude ultrastructurale de la dédifférenciation cellulaire dans la régénération antérieure de l'Annélide: Owenia fusiformis. Ann. Embryol. et Morph. 7, 61–79 (1974)

    Google Scholar 

  • Coulon, J., Marilley, M., Thouveny, Y.: Variations journalières du taux des sécrétions nerveuses chez Owenia fusiformis. Persistance d'un rythme journalier dans les tissus en culture. C.R. Acad. Sci., Paris 283, D, 1535–1538 (1976)

    Google Scholar 

  • Dahlström, A.: Aminergic transmission. Introduction and short review. Brain Res. 62, 441–460 (1972)

    Google Scholar 

  • Dainhaut-Courtois, N.: Etude en microscopic électronique et en fluorescence des médiateurs chimiques du système nerveux des Nereidae (Annélides Polychètes). Z. Zellforsch. 126, 90–103 (1970)

    Google Scholar 

  • Dainhaut-Courtois, N., Dhainaut, A.: Gamma-aminobutyric acid and 5-hydroxytryptamine in the nervous system of an annelid worm (Nereis diversicolor). J. Microsc. Biol. Cell 27, 261–266 (1976)

    Google Scholar 

  • Descarries, L., Droz, B.: Intraneural distribution of exogenous norepinephrine in the central nervous system of the rat. J. Cell Biol. 44, 385–399 (1970)

    Google Scholar 

  • Descarries, L., Dupin, J.C.: Retention of noradrenaline-3H in brain and preferential extraction of labeled metabolites by glutaraldehyde fixation. Experientia 30, 1164–1165 (1974)

    Google Scholar 

  • Franquinet, R., Stengel, D., Hanoune, J.: The adenylate cyclase system in a fresh-water planarian (Polycelis tenius IIJIMA). Comp. Biochem. Physiol. 53B, 329–333 (1976)

    Google Scholar 

  • Gerschenfeld, H.M.: Chemical transmission in invertebrate central nervous system and neuromuscular junctions. Physiol. Rev. 53, 1–119 (1973)

    Google Scholar 

  • Goh, S.L., Davey, K.G.: Selective uptake of noradrenaline, DOPA, and 5-hydroxytryptamine by the nervous system of Phocanema decipiens (Nematoda): a light autoradiographic and ultrastructural study. Tissue and Cell 8, 421–435 (1976)

    Google Scholar 

  • Goldman, J.E., Schwartz, J.H.: Metabolism of 3H-serotonin in the marine mollusc, Aplysia californica. Brain Res., 136, 77–88 (1977)

    Google Scholar 

  • Hamon, M.,: Biosynthèse de la sérotonine dans le système nerveux central. Quelques aspects de sa régulation. Thèse Dt es-sci. Nat. Paris VII (1975)

  • Hiripi, L., Salanki, J.: The role of monoamine-oxydase in the inactivation of serotonin in the nervous system and other tissues of Anodonta cygnea. Ann. Biol. tihany. 38, 31–38 (1971)

    Google Scholar 

  • Iversen, L.L.: Uptake mechanisms for neurotransmitter amines. Biochem. Pharmacol. 7, 1927–1935 (1974)

    Google Scholar 

  • Larra, F., Droz, B.: Techniques radioautographiques et leur application à l'étude du renouvellement des constituants cellulaires. J. Microscopic 9, 845–880 (1970)

    Google Scholar 

  • Manaranche, R.: Contribution à la connaissance du système nerveux de Glycera convoluta (K.) Annélide Polychète. Thèse Dt es-sci. Nat. Paris (1975)

  • Manaranche, R., L'Hermite, P.: Etude des aminés biogènes de Glycera convolute K. (Annélide Polychète). Z. Zellforsch. 137, 21–36 (1973)

    Google Scholar 

  • Pellegrino de Iraldi, A., Gueudet, R., Suburo, A.M.: Differentiation between 5-hydroxytryptamine and catecholamine in synaptic vesicles. Progr. Brain Res. 34, 161–170 (1971)

    Google Scholar 

  • Shaskan, E.G., Snyder, S.H.: Kinetics of serotonin accumulation into slices from rat brain: relationship to catecholamine uptake. J. Pharmacol. Exp. Therap. 175, 404–418 (1970)

    Google Scholar 

  • Sotelo, C.: The fine structural localization of norepinephrine-3H in the substantia nigra and area postrema of the rat. An autoradiographic study. J. Ultrastruct. Res. 36, 824–841 (1971)

    Google Scholar 

  • Ternaux, J.P., Hery, F., Bourgoin, S., Adrien, J., Glowinski, J., Hamon, M.: The topographical distribution of serotoninergic terminals in the neostriatum of the rat and the caudate nucleus of the cat. Brain Res. 121, 311–326 (1977)

    Google Scholar 

  • Thouveny, Y.,: Etude expérimentale et biochimique du fonctionnement des systèmes histogénétiques et de la dédifférenciation cellulaire dans la morphogenèse des Annélides Polychètes. Thèse Dt es-sci. Nat. Marseille (1967)

Download references

Author information

Authors and Affiliations

  1. Département de Biologie Cellulaire et Moléculaire, Laboratoire de Morphogénèse Animale, Marseille-Luminy

    J. Coulon & R. Bessone

  2. Département de Neurobiologie Cellulaire, Institut de Neurophysiologie et Psychophysiologie, Marseille, France

    J. Coulon & R. Bessone

Authors
  1. J. Coulon
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. R. Bessone
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

The authors thank Dr. A. Calas for useful discussions and critical reading of the manuscript, and G. Monty for his technical assistance. The investigations cited in the present paper were supported by a research grant n∘77.4.178.6 from the INSERM

Rights and permissions

Reprints and permissions

About this article

Cite this article

Coulon, J., Bessone, R. Autoradiographic detection of indolamine and catecholamine neurons in the nervous system of Owenia fusiformis (Polychaeta, Annelida). Cell Tissue Res. 198, 95–104 (1979). https://doi.org/10.1007/BF00234837

Download citation

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00234837

Key words

Search

Navigation