Abstract
Transgenic peanut plants were produced using Agrobacterium mediated gene transfer. Primary leaf explants of peanut were co-cultivated with Agrobacterium tumefaciens LBA 4404 harbouring the binary plasmid pBI 121 (conferring β-glucuronidase activity and resistance to kanamycin) and cultured on regeneration medium supplemented with kanamycin to select putatively transformed shoots. They were rooted and plants were transferred to soil. Stable integration and expression of the transgenes were confirmed by NPT II assay, Southern blot hybridization and GUS assay.
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Abbreviations
- BA:
-
6-benzyladenine
- GUS:
-
β-glucuronidase
- IAA:
-
indole-3-acetic acid
- NAA:
-
α-naphthaleneacetic acid
- NOS:
-
nopaline synthase
- NPT II:
-
neomycin phosphotransferase II
- SDS:
-
Lauryl sulfate
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Communicated by G. C. Phillips
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Eapen, S., George, L. Agrobacterium tumefaciens mediated gene transfer in peanut (Arachis hypogaea L.). Plant Cell Reports 13, 582–586 (1994). https://doi.org/10.1007/BF00234516
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DOI: https://doi.org/10.1007/BF00234516