Abstract
Hypocotylary explants obtained from 30- to 40-day-old slash pine (Pinus elliottii, Engelm.) seedlings treated with 6-benzylaminopurine produced multiple buds that eventually elongated into axillary shoots. The explants were pulse treated (45-s dip) with 6-benzylaminopurine (22.2, 111, 222 μM) plus a control and cultured on three different basal media containing activated charcoal (0.5% w/v). Hormonal concentration and basal medium were compared for the number and size of axillary shoots induced after 12 and 29 days. The greatest number of axillary shoots was produced by explants that were pulse treated with 111 μM 6-benzylaminopurine and cultured on Gresshoff and Doy medium. The axillary shoots were fewer in number per explant than shoots previously reported resulting from hormonally induced advantitious buds of slash pine, but the axillary shoots developed more rapidly.
Similar content being viewed by others
Abbreviations
- BA:
-
6-benzylaminopurine
- DMSO:
-
dimethyl sulfoxide
- PAR:
-
photosynthetically active radiation
References
Abdullah AA, Yeomen MM, Grace J (1989) In: Bajaj YPS (ed) Biotechnology in Agriculture and Forestry 5; Trees 2, Springer-Verlag, Berlin, pp 479–506.
Ahuja MR (1987) In: Bonga JM, Durzan DJ (eds) Cell and Tissue Culture in Forestry, vol I, Martinus Nijhoff/Dr W Junk, Netherlands, pp 272–285.
Amerson HV, Frampton LJ Jr, Mott RL, Spain PC (1988) In: Hanover JW, Keathley DE (eds) Genetic Manipulation of Woody Plants, Plenum Press, New York, pp 117–137.
Bruinsma J (1963) Photochem Photobiol 2:241–249.
Cheng T-Y (1977) Plant Sci Lett 9:179–187.
Lanner RM (1978) Forest Sci 24:167–179.
Lesney MS, Johnson JD, Korhnak T, McCaffery NW (1988) In: Hanover JW, Keathley DE (eds) Genetic Manipulation of Woody Plants, Plenum Press, New York, pp 43–55.
Mott RL, Smeltzer RH, Mehra-Palta A, Zobel BJ (1977) Tappi 60(7):62–64.
Perez-Bermudez P, Sommer HE (1987) Plant Cell, Tissue and Organ Culture 11:25–35.
Romberger JA (1963) Meristems, Growth, and Development in Woody Plants, US Dept Ag, FS Tech Bulletin No 1293.
Sacher JA (1955) Am J Bot 42:784–792.
Schlarbaum SE (1988) In: MR Ahuja (ed) Somatic Cell Genetics of Woody Plants, M Nijhoff, Netherlands, pp 103–118.
Schwab DW, Rizert RW (1970) Am J Bot 57:738.
Sokal RR, Rohlf FJ (1981) Biometry: The Principles and Practices of Statistics in Biological Research, 2nd ed, WH Freeman, New York.
Sommer HE, Brown CL (1974) Am J Bot 61(suppl 5):11.
Sommer HE, Brown CL, Kormanik PP (1975) Bot Gaz 136:196–200.
Timmis R, El-Nil A, Stonecypher RW (1987) In: Bonga JM, Durzan DJ (eds) Cell and Tissue Culture in Forestry, vol I, Martinis Nijhoff, Dordrecht, pp {198–215}.
Zel J, Gogala N, Camloh M (1988) Plant Cell, Tissue and Organ Culture 14:169–175.
Zobel B, Talbert J (1976) Applied Forest Tree Improvement, John Wiley & Sons, New York.
Author information
Authors and Affiliations
Additional information
Communicated by A. R. Gould
Rights and permissions
About this article
Cite this article
Burns, J.A., Schwarz, O.J. & Schlarbaum, S.E. Multiple shoot production from seedling explants of slash pine (Pinus elliottii, Engelm.). Plant Cell Reports 10, 439–443 (1991). https://doi.org/10.1007/BF00233810
Received:
Revised:
Published:
Issue Date:
DOI: https://doi.org/10.1007/BF00233810