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High rate of virus-free plantlet regeneration via garlic scape-tip culture

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Abstract

Shoot cultures of Allium sativum L. were established from stem-tip and scape-tip. Various combinations of media, growth regulators were compared. The best establishment and proliferation of stem-tip and scape-tip explants were obtained on MS medium containing 0.5 mg l−1 NAA and 0.5 mg l−1 KT. The roots were produced and the bulblets were formed after the micro-shoots were transferred to the hormone-free MS medium. The virus of each tube-plant was detected by ELISA. The virus elimination rates of plantlets from stem-tip with 3 leaf primordia, with 1–2 leaf primordia, and scape-up were 26.5%, 45.4%, and 77.6% respectively. The procedure of scape-tip culture would be applied for virus elimination to the species propagated vegetatively.

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Author notes

  1. Hong-Long Wang

    Present address: INRA, Symbiotes des Racines, 34060, Montpellier, France

Authors and Affiliations

  1. Department of Biology, Tianjin Normal University, 300074, Tianjin, China

    Yun Ma & Hong-Long Wang

  2. Center of Laboratories, Tianjin Institute of Education, 300020, Tianjin, China

    Cun-Jin Zhang & Yu-Qing Kang

Authors
  1. Yun Ma
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  2. Hong-Long Wang
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  3. Cun-Jin Zhang
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  4. Yu-Qing Kang
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Communicated by A. Komamine

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Ma, Y., Wang, HL., Zhang, CJ. et al. High rate of virus-free plantlet regeneration via garlic scape-tip culture. Plant Cell Reports 14, 65–68 (1994). https://doi.org/10.1007/BF00233301

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  • DOI: https://doi.org/10.1007/BF00233301

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