Summary
Somatic embryogenesis was obtained from hypocotyls and cotyledons of one month old plantlets of Picea abies. Embryogenic yield was higher with expiants from somatic embryo-derived plantlets (80 %) than with plantlets issued from zygotic embryos (10 %). This report also describes production of embryogenic calli from needles of 14 month old somatic embryo-derived plants cultivated in greenhouse. The influence of the physiological status and genotype of the mother plant on somatic embryogenic potential is discussed.
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Abbreviations
- ABA:
-
abscisic acid
- (±) ABA:
-
racemic ABA
- BAP:
-
6-benzylaminopurine
- CI:
-
callus inducing culture medium
- NAA:
-
1-naphtaleneacetic acid
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Communicated by G. Pelletier
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Ruaud, JN., Bercetche, J. & Pâques, M. First evidence of somatic embryogenesis from needles of 1-year-old Picea abies plants. Plant Cell Reports 11, 563–566 (1992). https://doi.org/10.1007/BF00233093
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DOI: https://doi.org/10.1007/BF00233093