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Regeneration of protoplast-derived green plants of Kentucky blue grass (Poa pratensis L.)

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Summary

Green plants were repeatedly regenerated from suspension-derived protoplasts of Kentucky blue grass (Poa pratensis L.) cv. “Geronimo”. One suspension was capable of donating competent protoplasts during long-term culture i. e. 10–16 months after its establishment. The plating efficiency of the protoplasts from three different suspension lines varied from 0.004% in the lowest up to 1.5% in the highest responding line, using agarose-bedding in nutrition medium devoid of nurse or feeder cultures. Green plants germinated from polyembryos, which developed from 0.4–2.7% of the protoplast-derived microcolonies. A total of 127 plants were successfully transferred to soil.

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Abbreviations

ABA:

abscisic acid

BAP:

6-benzylaminopurine

2,4-D:

2,4-dichlorophenoxyacetic acid

FW:

fresh weight

PE:

plating efficiency

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Author notes

  1. Kirsten Annette Nielsen

    Present address: Laboratory for Gene Expression, Department of Molecular Biology, University of Aarhus, Gustav Wiedsvej 10, DK-8000, Århus C, Denmark

Authors and Affiliations

  1. Dansk Planteforædling A/S, Boelshøj, P.O. 19, DK-4660, St. Heddinge, Denmark

    Kirsten Annette Nielsen, Else Larsen & Elisabeth Knudsen

Authors
  1. Kirsten Annette Nielsen
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  2. Else Larsen
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  3. Elisabeth Knudsen
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Communicated by H. Lörz

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Nielsen, K.A., Larsen, E. & Knudsen, E. Regeneration of protoplast-derived green plants of Kentucky blue grass (Poa pratensis L.). Plant Cell Reports 12, 537–540 (1993). https://doi.org/10.1007/BF00233055

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  • DOI: https://doi.org/10.1007/BF00233055

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