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Organogenesis in Camellia x williamsii: cytokinin requirement and susceptibility to antibiotics

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Summary

The genetic transformation of plants calls for efficient organogenetic methods. Both cytokinins and antibiotics were tested to evaluate shoot regeneration from internodes of in vitro plants of Camellia x williamsii cv Debbie. High regeneration rates were achieved by using thidiazuron, which turned out to be more effective than 6-benzylaminopurine. Up to 96% of explants regenerated when thidiazuron was used, whereas no more than 75% regenerated using 6-benzylaminopurine. The best average number of shoots per regenerant explant was 9.7 and 5.6 regarding respectively thidiazuron and 6-benzylaminopurine. Kanamycin, used in combination with the best performing thidiazuron concentration, completely blocked regeneration at 129 μM. Cefotaxime at 524 μM decreased the regeneration ability, especially when 2 day preculture was applied. The application of genetic transformation protocols as well as the main aims of genetic engineering in ornamental camellias are discussed.

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Abbreviations

MS:

Murashige & Skoog

WPM:

Woody Plant Medium

BA:

6-benzylaminopurine

TDZ:

thidiazuron

IBA:

indole butyric acid

NPTII :

neomycin phosphotransferase II

CaMV:

Cauliflower Mosaic Virus

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Authors and Affiliations

  1. Fondazione Minoprio, Viale Raimondi, 54, 22070, Vertemate con Minoprio (Como), Italy

    A. Tosca, R. Pandolfi & S. Vasconi

Authors
  1. A. Tosca
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  2. R. Pandolfi
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  3. S. Vasconi
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Communicated by I. Potrykus

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Tosca, A., Pandolfi, R. & Vasconi, S. Organogenesis in Camellia x williamsii: cytokinin requirement and susceptibility to antibiotics. Plant Cell Reports 15, 541–544 (1996). https://doi.org/10.1007/BF00232990

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  • DOI: https://doi.org/10.1007/BF00232990

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