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Partial desiccation of mature embryo-derived calli, a simple treatment that dramatically enhances the regeneration ability of indica rice

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Abstract

Regeneration of indica rice varieties remains a limiting factor for researchers undertaking rice Iransformation experiments. As reported for japonica rice and other crops, partial desiccation of indica rice calli dramatically promotes organogenesis and leads to high regeneration ability. We are now able to obtain 66.5%, 61.1% and 73.7% of calli that regenerate plants for the indica varieties TN1, IR72 and IR64 whereas in non desiccated controls only 30.0%, 15.5% and 18.7% of calli regenerated, respectively. Plants obtained were phenotypically normal and 50% were highly fertile. Partial desiccation is a reliable and simple method for improving indica rice regeneration. It also shortens the time of in vitro culture.

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Abbreviations

2,4-D:

2,4-Dichlorophenoxyacetic acid

BAP:

6-Benzyl amino purine

DTT:

Dithiothreitol

EDTA:

Ethylene diamine tetra-acetic acid

MS:

Murashige and Skoog

NAA:

Naphtalene acetic acid

PAGE:

Polyacrylamide gel electrophoresis

PAR:

Photosynthetic active radiation

SDS:

Sodium dodecil sulfate

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Author notes

  1. Iann M. Rancé

    Present address: Université Paul Sabatier, Centre de Physiologie Végétale, 118 route de Narbonne, 31062, Toulouse Cedex, France

Authors and Affiliations

  1. ILTAB/ORSTOM Division of Plant Biology-MRC7, 10666 North Torrey Pines Road, 92037, La Jolla, CA, USA

    Iann M. Rancé, Alexandre de Kochko & Claude Fauquet

  2. ILTAB/TSRI, Division of Plant Biology-MRC7, 10666 North Torrey Pines Road, 92037, La Jolla, CA, USA

    Wenzhong Tian, Helena Mathews & Roger N. Beachy

Authors
  1. Iann M. Rancé
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  2. Wenzhong Tian
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  3. Helena Mathews
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  4. Alexandre de Kochko
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  5. Roger N. Beachy
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  6. Claude Fauquet
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Additional information

Communicated by I. K. Vasil

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Rancé, I.M., Tian, W., Mathews, H. et al. Partial desiccation of mature embryo-derived calli, a simple treatment that dramatically enhances the regeneration ability of indica rice. Plant Cell Reports 13, 647–651 (1994). https://doi.org/10.1007/BF00232938

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  • DOI: https://doi.org/10.1007/BF00232938

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