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Transient expression of β-glucuronidase following biolistic delivery of foreign DNA into coffee tissues

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Abstract

Some conditions related to the transient expression of β-glucuronidase in biolistically-treated Coffea spp. tissues were investigated, and subsequently used in a promoter study. Bombardments were performed on different types of tissue (leaves, somatic embryos and suspension cultures) of genotypes of C. arabica, C. canephora and Arabusta, using 4 different promoter sequences. Tobacco leaves were used as a comparison. In general, similar large variation and mean values of transient expression were observed between coffee and tobacco leaves. With regard to the coffee tissues effect, transient expression was best detectable and most frequently observed with bombarded leaves of microcuttings. Disturbing endogenous light blue staining was found with control treatments of somatic embryos. For the three coffee species tested, the most effective promoter was the EF1α-A1 promoter of Arabidopsis thaliana.

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Abbreviations

BA:

6-benzylaminopurine

EDTA:

ethylenediaminetetraacetic acid

GUS:

β-glucuronidase

HFSE:

high frequency somatic embryogenesis

IAA:

indole-3-acetic acid

IBA:

indole-3-butyric acid

MS:

Murashige & Skoog (1962)

PVP:

polyvinylpyrrolidone

R:

regeneration; rpm, rotations per minute

SAS:

statistical analytical system

v/v:

volume per volume

w/v:

weight per volume

X-gluc:

5-bromo-4-chloro-3-indolyl-β-D-glucuronic acid

2,4-D:

2,4-dichlorophenoxyacetic acid

2-iP:

2-isopentenyladenine

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Author notes

  1. Jos van Boxtel

    Present address: Department of Virus Research, John Innes Centre, Colney Lane, NR4 7UH, Norwich, UK

Authors and Affiliations

  1. CIRAD-CP, B.P. 5035, F-34032, Montpellier cedex 1, France

    Jos van Boxtel, Marc Berthouly, Cathy Carasco & Albertus Eskes

  2. CATIE, 7170, Turriaiba, Costa Rica

    Magali Dufour

Authors
  1. Jos van Boxtel
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  2. Marc Berthouly
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  3. Cathy Carasco
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  4. Magali Dufour
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  5. Albertus Eskes
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Communicated by A. M. Boudet

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van Boxtel, J., Berthouly, M., Carasco, C. et al. Transient expression of β-glucuronidase following biolistic delivery of foreign DNA into coffee tissues. Plant Cell Reports 14, 748–752 (1995). https://doi.org/10.1007/BF00232915

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  • DOI: https://doi.org/10.1007/BF00232915

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