Summary
Variations in the ploidy level of 69 transgenic potato (Solanum tuberosum L.) plants regenerated from the tuber discs of 17 diploid lines were studied: 24 plants (35%) were diploid, the other 45 plants (65%) were tetraploid. Seventy-eight control regenerants obtained without Agrobacterium inoculation showed a relatively low tendency to tetraploidization (35%). The results obtained suggested that chromosome doubling occurred frequently in diploid potato lines during the tissue culture process for regeneration. Putative somaclonal changes in in vitro-formed tuber proteins were detected in three out of six transformants by electrophoretic analysis.
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Communicated by M. Tabata
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Imai, T., Aida, R. & Ishige, T. High frequency of tetraploidy in Agrobacterium-mediated transformants regenerated from tuber discs of diploid potato lines. Plant Cell Reports 12, 299–302 (1993). https://doi.org/10.1007/BF00237422
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DOI: https://doi.org/10.1007/BF00237422