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Choleragenoid horseradish peroxidase used for studying projections of some hindlimb cutaneous nerves and plantar foot afferents to the dorsal horn and Clarke's column in the rat

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Summary

The aim of the present study has been to investigate the spinal projections of cutaneous hindlimb afferents particularly to the deep dorsal horn and to Clarke's column (CC), by using the B-subunit of cholera toxin conjugated to horseradish peroxidase. Injections into three different cutaneous hindlimb nerves in adult rats resulted in dense labeling in the dorsal horn laminae IIi-IV/V, moderate labeling in lamina I and modest labeling in dorsomedial parts of CC. Footpad injections gave similar results, except for a lack of labeling in CC and only weak labeling in laminae I and V. The results suggest that B-HRP should be a useful marker for studying cutaneous myelinated nerve fiber projections to the rat spinal cord.

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References

  • Boehme CC (1968) The neural structure of Clarke's nucleus of the spinal cord. J Comp Neurol 132:445–462

    Google Scholar 

  • Brown AG (1981) Organization in the spinal cord. Springer, Berlin Heidelberg New York

    Google Scholar 

  • Brown PB, Culberson JL (1981) Somatotopic organization of hindlimb cutaneous dorsal root projections to cat dorsal horn. J Neurophysiol 45:137–143

    CAS  PubMed  Google Scholar 

  • Brown PB, Fuchs JL (1975) Somatotopic representation of hindlimb skin in cat dorsal horn. J Neurophysiol 38:1–9

    Google Scholar 

  • Brown AG, Rose PK, Snow PJ (1977) The morphology of hair follicle afferent fibre collaterals in the spinal cord of the cat. J Physiol (Lond) 272:779–797

    Google Scholar 

  • Brown AG, Rose PK, Snow PJ (1978) Morphology and organization of axon collaterals from afferent fibres of slowly adapting type I units in cat spinal cord. J Physiol (Lond) 277:15–27

    Google Scholar 

  • Brown AG, Fyffe REW, Noble R (1980) Projections from Pacinian corpuscles and rapidly adapting mechanoreceptors of glabrous skin to the cat's spinal cord. J Physiol (Lond) 307:385–400

    Google Scholar 

  • Craig AD, Mense S (1983) The distribution of afferent fibers from the gastrocnemius-soleus muscle in the dorsal horn of the cat, as revealed by the transport of horseradish peroxidase. Neurosci Lett 41:233–238

    Google Scholar 

  • Ganser AL, Kirschner DA, Willinger M (1983) Ganglioside localization on myelinated nerve fibres by cholera toxin binding. J Neurocytol 12:921–938

    Google Scholar 

  • Hebel R, Stromberg MW (1986) Anatomy and embryology of the laboratory rat. BioMed, Wörthsee

    Google Scholar 

  • Koerber HR, Brown PB (1982) Somatotopic organization of hindlimb cutaneous nerve projections to cat dorsal horn. J Neurophysiol 48:481–489

    Google Scholar 

  • Kumazawa T, Pearl ER (1978) Excitation of marginal and substantia gelatinosa neurons in the primate spinal cord: Indications of their place in dorsal horn functional organization. J Comp Neurol 177:417–434

    Google Scholar 

  • LaMotte C (1977) Distribution of the tract of Lissauer and the dorsal root fibers in the primate spinal cord. J Comp Neurol 172:529–562

    Google Scholar 

  • Light AR, Durkovic RG (1984) Features of laminar and somatotopic organization of lumbar spinal cord units receiving cutaneous inputs from hindlimb receptive fields. J Neurophysiol 52:449–458

    Google Scholar 

  • Light AR, Pearl ER (1977) Differential termination of large diameter and small diameter primary afferent fibers in the spinal dorsal gray matter as indicated by labeling with horseradish peroxidase. Neurosci Lett 6:59–63

    Google Scholar 

  • Light AR, Perl ER (1979a) Reexamination of the dorsal root projection to the spinal dorsal horn including observation on the differential termination of coarse and fine fibers. J Comp Neurol 186:117–132

    Google Scholar 

  • Light AR, Perl ER (1979b) Spinal termination of functionally identified primary afferent neurons with slowly conducting myelinated fibers. J Comp Neurol 186:133–150

    Google Scholar 

  • Mense S, Craig AD (1988) Spinal and supraspinal terminations of primary afferent fibers from the gastrocnemius-soleus muscle in the cat. Neurosci 26:1023–1035

    Google Scholar 

  • Mesulam M-M, Hegarty E, Barbas H, Carson KA, Gower EC, Knapp AG, Moss MB, Mufson EJ (1980) Additional factors influencing sensitivity in the tetrametylbenzidine method for horseradish peroxidase neurohistochemistry. J Histochem Cytochem 28:1255–1259

    Google Scholar 

  • Meyers DER, Snow PJ (1984) Somatotopically inappropriate projections of single HFA's to cat spinal cord. J Physiol (Lond) 347:59–73

    Google Scholar 

  • Molander C, Grant G (1985) Cutaneous projections from the rat hindlimb foot to the substantia gelatinosa of the spinal cord studied by transganglionic transport of WGA-HRP conjugate. J Comp Neurol 237:476–484

    Google Scholar 

  • Molander C, Grant G (1986) Laminar distribution and somatotopic organization of primary afferent fibers from hindlimb nerves in the dorsal horn. A study by transganglionic transport of horseradish peroxidase in the rat. Neuroscience 19:297–312

    Google Scholar 

  • Molander C, Xu Q, Grant G (1984) The cytoarchitectonic organization of the spinal cord in the rat. I. The lower thoracic and lumbosacral cord. J Comp Neurol 230:133–141

    Google Scholar 

  • Nyberg G, Blomqvist A (1985) Somatotopic organization of the forelimb cutaneous nerves in brachial dorsal horn: an anatomical study in the cat. J Comp Neurol 242:28–39

    Google Scholar 

  • Pomeranz B, Wall PD, Weber WV (1968) Cord cells responding to fine myelinated afferents from viscera, muscle and skin. J Physiol (Lond) 199:511–532

    Google Scholar 

  • Randíc M, Myslinski NR, Gordon JH (1976) Spinal localization of neurons receiving inputs from cutaneous afferents in the cat hindlimb. Brain Res 105:573–577

    Google Scholar 

  • Réthelyi M (1968) The Golgi architecture of Clarke's column. Acta Morphol Hung 16:311–330

    Google Scholar 

  • Réthelyi M (1977) Preterminal and terminal axon arborizations in the substantia gelatinosa of cat's spinal cord. J Comp Neurol 172:511–528

    Google Scholar 

  • Rivero-Melián C, Grant G (1988) Somatotopic organization of hindlimb muscle afferent projections to the column of Clarke of the rat studied with choleragenoid horseradish peroxidase conjugate. Soc Neurosci Abstr 14:1:693

    Google Scholar 

  • Rivero-Melián C, Grant G (1990a) Lumbar dorsal root projections to spinocerebellar cell groups in the rat spinal cord: a double labeling study. Exp Brain Res 81:85–94

    Google Scholar 

  • Rivero-Melián C, Grant G (1990b) Distribution of lumbar dorsal root fibers in the lower thoracic and lumbosacral spinal cord of the rat studied with choleragenoid horseradish peroxidase conjugate. J Comp Neurol 299:470–481

    Google Scholar 

  • Robertson B, Arvidsson J (1985) Transganglionic transport of wheat germ agglutinin-HRP and choleragenoid-HRP in rat trigeminal primary sensory neurons. Brain Res 348:44–51

    Google Scholar 

  • Robertson B, Grant G (1985) A comparison between wheat germ agglutinin and choleragenoid-horseradish peroxidase as anterogradely transported markers in central branches of primary sensory neurons in the rat with some observations in the cat. Neurosci 14:895–905

    Google Scholar 

  • Robertson B, Grant G (1989) Immunocytochemical evidence for the localization of the GM1 ganglioside in carbonic anhydrase-containing and RT 97-immunoreactive rat primary sensory neurons. J Neurocytol 18:77–86

    Google Scholar 

  • Shortland P, Woolf CJ, Fitzgerald M (1989) Morphology and Somatotopic organization of the central terminals of hindlimb hair follicle afferents in the rat lumbar spinal cord. J Comp Neurol 289:416–433

    Google Scholar 

  • Sugiura Y, Lee CL, Perl ER (1986) Central projections of identified, unmyelinated (C) afferent fibers innervating mammalian skin. Science 234:358–361

    Google Scholar 

  • Swett JE, Woolf CJ (1985) The somatotopic organization of primary afferent terminals in the superficial laminae of the dorsal horn of the rat spinal cord. J Comp Neurol 231:66–77

    Google Scholar 

  • Wall PD (1967) The laminar organization of dorsal horn and effects of descending impulses. J Physiol (Lond) 192:123–144

    Google Scholar 

  • Woolf CJ (1987) Central terminations of cutaneous mechanoreceptive afferents in the rat lumbar spinal cord. J Comp Neurol 261:105–119

    Google Scholar 

  • Woolf CJ, Fitzgerald M (1986) Somatotopic organization of cutaneous afferent terminals and dorsal horn neuronal receptive fields in the superficial and deep laminae of the rat lumbar spinal cord. J Comp Neurol 251:517–531

    Google Scholar 

  • Ygge J, Grant G (1983) The organization of the thoracic spinal nerve projection in the rat dorsal horn demonstrated with transganglionic transport of Horseradish peroxidase. J Comp Neurol 216:1–9

    Google Scholar 

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  1. Department of Anatomy, Karolinska Institute, Box 60 400, S-104 01, Stockholm, Sweden

    C. Rivero-Melián & G. Grant

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  1. C. Rivero-Melián
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  2. G. Grant
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Rivero-Melián, C., Grant, G. Choleragenoid horseradish peroxidase used for studying projections of some hindlimb cutaneous nerves and plantar foot afferents to the dorsal horn and Clarke's column in the rat. Exp Brain Res 84, 125–132 (1991). https://doi.org/10.1007/BF00231767

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  • DOI: https://doi.org/10.1007/BF00231767

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