Summary
It is shown that in vitro pollination can be used in future studies of the time course of pollen-tube development and analysis of self-incompatibility in sugar beet, Beta vulgaris L. Upon selfng a self-incompatible genotype showed the same incompatibility response after both in vitro and in vivo pollinations. No differences between cross-compatible and self-compatible pollentube growth were observed. The pollen-tube rejection occurred whether or not the pollen was prehydrated. RNA staining with Acridine Orange showed that there was less cellular RNA in the pistil tissue from in vitro-pollinated flowers. Nevertheless, pollen-tube growth and the self-incompatibility response were similar after in vivo and in vitro pollinations.
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Haldrup, A., Bruun, L. Self-incompatibility reactions and compatible pollen-tube growth are retained with in vitro pollinations of sugar beet, Beta vulgaris L.. Sexual Plant Reprod 6, 46–51 (1993). https://doi.org/10.1007/BF00227582
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DOI: https://doi.org/10.1007/BF00227582