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Characteristic induction of 70 000 Da-heat shock protein and metallothionein by zinc in HeLa cells

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Abstract

The synthesis of a 70 000 dalton-heat shock protein (hsp70) is one of several heat shock proteins induced in HeLa cells during the incubation in medium containing zinc sulphate. The synthesis of hsp70 was increased in the presence of 200 μM zinc sulphate and above, but not at 100 μM zinc sulphate. On the other hand, the synthesis of metallothionein was activated in the presence of 100 μM zinc sulphate and above. Uptake of zinc into the cells depended on the concentration of zinc sulphate in the medium. The separation of intracellular zinc into three fractions by gel filtration chromatography; high molecular, metallothionein, and low molecular fractions, showed that zinc in the low molecular weight and metallothionein fractions was elevated in the presence of 100 μM zinc sulphate in the medium, whereas increase in the zinc content of the high molecular weight fraction occurred at 200 μM zinc sulphate and above. Inhibition of cell growth and cellular protein synthesis was also observed at 200 μM zinc sulphate and above, but not at 100 μM. From these findings, since the induction of hsp70 synthesis and inhibition of cell growth occurred concomitantly with the increase of zinc in the high and low molecular weight fractions, hsp70 seemed not to function in the detoxification of zinc, but it may participate in the repair of zinc-induced damage.

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Authors and Affiliations

  1. Department of Biochemistry, Kyoto Pharmaceutical University, 5 Nakauchi-cho, Misasagi, Yamashina-ku, 607, Kyoto, Japan

    Takumi Hatayama, Yasuhiro Tsukimi, Tohru Wakatsuki, Teruko Kitamura & Hirotsugu Imahara

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  1. Takumi Hatayama
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  2. Yasuhiro Tsukimi
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  3. Tohru Wakatsuki
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  4. Teruko Kitamura
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  5. Hirotsugu Imahara
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Hatayama, T., Tsukimi, Y., Wakatsuki, T. et al. Characteristic induction of 70 000 Da-heat shock protein and metallothionein by zinc in HeLa cells. Mol Cell Biochem 112, 143–153 (1992). https://doi.org/10.1007/BF00227571

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  • DOI: https://doi.org/10.1007/BF00227571

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