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Processing of antennular input in the brain of the spiny lobster, Panulirus argus

II. The olfactory pathway

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Abstract

Neurons in the olfactory deutocerebrum of the spiny lobster, Panulirus argus, were recorded intracellularly and filled with biocytin. Recorded neurons arborized in the olfactory lobe (OL), a glomerular neuropil innervated by olfactory and some presumptive mechanosensory antennular afferents. The neurons responded to chemosensory input from the lateral antennular flagellum bearing the olfactory sensilla but not the medial flagellum bearing many non-olfactory chemosensory sensilla. Many neurons received additional mechanosensory input. Thus the OL integrates specifically olfactory with mechanosensory input. OL neurons had multiglomerular arborizations restricted to one or two of the three horizontal layers of the columnar glomeruli. OL local interneurons comprised “core” neurons with tree-like neurites and terminals in the base of the glomeruli and “rim” neurons with neurites surrounding the OL and terminals in the cap/subcap. The somata of OL local interneurons lay in the medial soma cluster (100000 somata). OL projection neurons arborized in the base of the glomeruli and ascended via the olfactory glomerular tract to the lateral protocerebrum. A parallel projection pathway is constituted by projection neurons of the accessory lobe, a glomerular neuropil without afferent innervation but intimate links to the OL. The projection neuron somata constituted the lateral soma cluster (200000 somata).

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Abbreviations

AC :

anterior cluster (cluster 6,7)

AL :

accessory lobe

aMC :

anterior subcluster of medial cluster (cluster 9)

A lNv:

main antenna I (antennular) nerve

A lNM:

antenna I (antennular) motor nerve

A llNv:

main antenna II (antennal) nerve

CB :

central body

CL :

central layer of accessory lobe

DC :

deutocerebral commissure

DCN :

deutocerebral commissure neuropil

dDUMC :

dorsal subcluster of dorsal unpaired median cluster (cluster 17)

dMC :

dorsal subcluster of medial cluster (cluster 11)

dVPALC :

dorsal subcluster of ventral paired anterolateral cluster (cluster 8) G glomerulus

IDUMC :

lateral subcluster of dorsal unpaired median cluster (cluster 16)

LC :

lateral cluster (cluster 10)

LF :

lateral flagellum of antenna I (antennule)

LL :

lateral layer of accessory lobe

MF :

medial flagellum of antenna I (antennule)

ML :

medial layer of accessory lobe

MPN :

anterior and posterior median protocerebral neuropils

OGT :

olfactory globular tract

OGTN :

olfactory globular tract neuropil

OL :

olfactory lobe

OLALT :

olfactory lobe-accessory lobe tract

PB :

protocerebral bridge

pMC :

posterior subcluster of medial cluster (cluster 9)

PT :

protocerebral tract

TNv :

tegumentary nerve

VPMC :

ventral paired medial cluster (cluster 12)

VUMC :

ventral unpaired medial cluster (cluster 13)

vVPALC :

ventral subcluster of ventral paired anterolateral cluster (cluster 8)

ASW :

artificial sea water

M3 :

mixture 3

PRO :

L-proline

TM :

TetraMarin extract

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Authors and Affiliations

  1. Institut für Biologie, TU Berlin, Franklinstraße 28/29, D-10587, Berlin, Germany

    M. Schmidt

  2. Whitney Laboratory, University of Florida, 32086, St. Augustine, FL, USA

    M. Schmidt & B. W. Ache

  3. Departments of Zoology and Neuroscience, University of Florida, 32086, St. Augustine, FL, USA

    B. W. Ache

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Schmidt, M., Ache, B.W. Processing of antennular input in the brain of the spiny lobster, Panulirus argus . J Comp Physiol A 178, 605–628 (1996). https://doi.org/10.1007/BF00227375

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