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Efficient extraction of RNA from mammalian tissue

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Summary

RNA extraction from mammalian tissue has been compared using the different deproteinizing agents: a) guanidine-HCl, b) guanidinium-thiocyanate, c) buffer-saturated phenol, or d) buffer-saturated phenol followed by a proteinase K digestion of the aqueous phase. Both solid tissues (first, second, and third trimester fetal bovine pancreas), and human white blood cell populations were studied. Degradation, as seen in citric acid-urea agarose gels, and the ability to serve as templates for cell-free protein synthesis were used as criteria to assess the efficiency of the different methods.

We conclude that employing buffer-saturated phenol with proteinase K digestion is a superior method for consistent extraction of relatively undegraded RNA in quantitative amounts from mammalian tissue.

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Authors and Affiliations

  1. Department of Biochemistry, The University of Texas System Cancer Center, M. D. Anderson Hospital and Tumor Institute, 77030, Houston, TX, USA

    Marsha L. Frazier, Wendy Mars, Dagne L. Florine, Richard A. Montagna & Grady F. Saunders

  2. The University of Texas Graduate School of Biomedical Science, 77030, Houston, TX, USA

    Marsha L. Frazier, Wendy Mars, Dagne L. Florine, Richard A. Montagna & Grady F. Saunders

Authors
  1. Marsha L. Frazier
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  2. Wendy Mars
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  3. Dagne L. Florine
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  4. Richard A. Montagna
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  5. Grady F. Saunders
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Frazier, M.L., Mars, W., Florine, D.L. et al. Efficient extraction of RNA from mammalian tissue. Mol Cell Biochem 56, 113–122 (1983). https://doi.org/10.1007/BF00227211

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  • DOI: https://doi.org/10.1007/BF00227211

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