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Purification of Total RNA from Stomach Tissue Biopsies

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Helicobacter Pylori

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2283))

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Abstract

In order to further our understanding of the physiological consequences of Helicobacter pylori infection , analysis of clinical tissue specimens is required. To this end, RNA is frequently isolated from stomach biopsies of H. pylori-infected patients and compared to samples from uninfected controls to monitor gene expression using molecular methods such as reverse-transcription real-time PCR, microarrays, and next-generation sequencing. The successful purification of sufficient quantities of high-quality RNA is essential for accurate and reproducible downstream analysis. This chapter describes the key steps for high-quality RNA purification from human tissue samples, including sample collection and storage, tissue disruption and lysis, RNA purification, and assessment of RNA yield and quality.

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Acknowledgments

Acknowledgements Support is acknowledged from the Health Research Board and a Trinity College Dublin Provost PhD Award.

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Authors and Affiliations

  1. School of Medicine, Trinity College Dublin, Dublin, Ireland

    Rebecca FitzGerald, Malini Devapal & Deirdre McNamara

  2. School of Pharmacy and Pharmaceutical Sciences, Trinity College Dublin, Dublin, Ireland

    John J. Hickey

  3. Faculty of Medicine, University of British Columbia, Vancouver, Canada

    Dermot Kelleher

  4. Department of Clinical Medicine, School of Medicine, Trinity College Dublin, Dublin 2, Ireland

    Sinéad M. Smith

Authors
  1. Rebecca FitzGerald
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  2. Malini Devapal
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  3. John J. Hickey
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  4. Deirdre McNamara
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  5. Dermot Kelleher
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  6. Sinéad M. Smith
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Corresponding author

Correspondence to Sinéad M. Smith .

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Editors and Affiliations

  1. Department of Clinical Medicine, School of Medicine, Trinity College Dublin, Dublin 2, Ireland

    Sinead M Smith

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FitzGerald, R., Devapal, M., Hickey, J.J., McNamara, D., Kelleher, D., Smith, S.M. (2021). Purification of Total RNA from Stomach Tissue Biopsies. In: Smith, S.M. (eds) Helicobacter Pylori. Methods in Molecular Biology, vol 2283. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1302-3_7

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  • DOI: https://doi.org/10.1007/978-1-0716-1302-3_7

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1301-6

  • Online ISBN: 978-1-0716-1302-3

  • eBook Packages: Springer Protocols

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