Summary
This paper reports the application of the RAPD (random amplification of polymorphic DNA sequence) markers in Brassica genetics. Forty-seven arbitrary decamer oligonucletides were used as primers to amplify genomic DNA by polymerase chain reaction. Some of the amplified products were genome specific and could be found in both diploid and derived amphidiploid species. Of a total of 65 such markers, 16 were A genome, 37 B genome, and 12 C genome specific. Of the 37 B-genome-specific markers, 11 were mapped on four independent chromosomes of B. nigra with the aid of existing B. napus-nigra disomic alien addition lines.
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References
Attia T, Röbbelen C (1986) Cytogenetic relationship within cultivated Brassica analyzed in amphihaploids from the three diploid ancestors. Can J Genet 28:322–329
Chevre AM, This P, Eber F, Deschamps M, Delseny M, Quiros CF (1991) Characterization of disomic addition lines Brassica napus-Brassica nigra by isozyme, fatty acids and RFLP markers. Theor Appl Gent 81:93–99
Hosaka K, Kianian SF, McGrath JM, Quiros CF (1990) Development and chromosomal localization of genome-specific DNA markers of Brassica and the evolution of amphidiploids and n=9 diploid species. Genome 33:1312–142
Hu J, Quiros CF (1991) Molecular and cytological evidence of deletions in alien chromosomes for two monosomic addition lines of Brassica campestris-oleracea. Theor Appl Genet 81:221–226
Janhier J, Chevre AM, Tanguy AM, Eber F (1989) Extraction of disomic addition lines of Brassica napus-B. nigra. Genome 32:408–413
Karpechenko GD (1922) The number of chromosomes and the genetic correlation of cultivated Cruciferae. Bull Appl Bot Genet Plant Breed 13:3–14
Ledbetter SA, Nelson DL, Warren ST, Ledbetter DH (1990) Rapid isolation within specific chromosome regions by interspersed repetitive sequence polymerase chain reaction. Genomics 6:475–481
Love JM, Knight AM, McAleer MA, Todd JA (1990) Toward the construction of a high resolution map of the mouse genome using PCR-analysed micro satellites. Nucleic Acid Res 18:4123–4130
McGrath JM, Quiros CF (1990) Generation of alien chromosome addition lines from synthetic Brassica napus: morphology, cytogenetics, fertility and chromosome transmission. Genome 33:374–383
McGrath JM, Quiros CF, Harada JJ, Landry BS (1990) Identification of Brassica oleracea monosomic alien chromosome addition lines with molecular markers reveals extensive gene duplication. Mol Gen Genet 223:198–204
Palmer JD, Shield CR, Cohen DB, Orton TJ (1983) Chloroplast DNA evolution and the origin of amphidiploid Brassica species. Theor Appl Genet 65:181–189
Quiros CF, Ochoa O, Kianian SF, Douches D (1987) Analysis of the Brassica oleracea genome by generation of B. campestris-oleracrea chromosome addition lines: characterization by isozymes and rDNA genes. Theor Appl Genet 74:758–766
Quiros CF, McGrath JM, Kianian SF, This P, Ochoa O (1989) Cytogenetic stocks in Brassica: Addition lines and genome evolution. In: Quiros CF, McGuire PE (eds) Proc 5th Crucifer Genet Workshop, University of California, Davis. Report of University of California Genetics Resources Conservation Program
Saiki RK, Gelfand DH, Stoffel S, Scharf S, Higuchi RH, Horn GT, Mullis KB, Erlich HA (1988) Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science 239:487–491
Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual, 2nd edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor/NY
Song KM, Osborn TC, Williams PH (1988) Brassica taxonomy based on nuclear restriction fragment length polymorphisms (RFLP's). 2. Preliminary analysis of subspecies within B. rapa (Syn. campestris) and B. oleracea. Theor Appl Genet 76:593–600
This P, Ochoa O, Quiros CF (1990) Dissection of the Brassica nigra genome by monosomic addition lines. Plant Breed 105:211–220
UN (1935) Genomic analysis in Brassica with special reference to the experimental formation of B. napus and peculiar mode of fertilization. Jpn J Bot 7:389–452
Williams JG, Kubelik AE, Levak KJ, Rafalski JA, Tingey SC (1991) DNA polymorphism amplified by arbitrary primers are useful as genetic markers. Nucleic Acid Res 18:6531–6535
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Communicated by H. F. Linskens
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Quiros, C.F., Hu, J., This, P. et al. Development and chromosomal localization of genome-specific markers by polymerase chain reaction in Brassica . Theoret. Appl. Genetics 82, 627–632 (1991). https://doi.org/10.1007/BF00226801
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DOI: https://doi.org/10.1007/BF00226801