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Effect of cyproterone acetate on cells of the pars distalis of the adenohypophysis in the Beagle Bitch

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Summary

The effects of oral administration of 100 mg per kg per day cyproterone acetate (CPA) for four weeks on cells of the pars distalis, as revealed by the immunoperoxidase technique and chemical staining, were studied in the ovariectomized beagle bitch. For immunochemical staining antisera to the following hormones were used: canine GH, canine PRL, porcine ACTH, bovine TSHβ, bovine LHβ and human FSHβ1. The most striking effects of the treatment were an overall increase in the relative proportion of GH cells and a marked morphological indication of high secretory activity in these cells. In contrast, PRL cells were not affected significantly. In all ovariectomized control bitches a marked atrophy of the cells stained for FSHβ (FSH cells) and hypertrophy of the cells shown to contain LHβ(LH cells) were observed. FSH cells became enlarged, while LH cells appeared reduced in size by administration of CPA. In some treated bitches ACTH/MSH cells showed atrophy and regressive changes, whereas TSH cells seemed to become enlarged and were more densely arranged. These structural responses indicate that, in addition to its partial antigonadotropic properties, CPA as a synthetic progesterone derivative may stimulate GH secretion and possibly suppress CRH-ACTH activity in the ovariectomized beagle bitch.

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Abbreviations

ACTH :

Adrenocorticotropin

FSH :

Follicle Stimulating Hormone

GH :

Growth Hormone

LH :

Luteinizing Hormone

MSH :

Melanocyte Stimulating Hormone

PRL :

Prolactin

TSH :

Thyrotropin

CRH :

Corticotropin Releasing Hormone

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Author notes

  1. M. R. Fath El Bab

    Present address: Department of Veterinary Anatomy, University of Assiut, Assiut, Egypt

Authors and Affiliations

  1. Research Laboratories of Schering AG, Berlin/Bergkamen, Federal Republic of Germany

    M. F. El Etreby

  2. Anatomisches Institut der Tierärztlichen Hochschule, Hannover, Federal Republic of Germany

    M. R. Fath El Bab

Authors
  1. M. F. El Etreby
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  2. M. R. Fath El Bab
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Additional information

The authors are grateful to Dr. Christel Schoebel and Mrs. P. Kurth for carrying out the experimental work on animals, to Dr. H. Wiemann for the statistical analysis, to Mrs. B. Schilk, G. Soulioti and Miss U. Tüshaus for their excellent technical assistance, and to Dr. P. Guenzel for his advice and encouragement

Recipient of a Research Scholarship from the Arabic Republic of Egypt

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El Etreby, M.F., El Bab, M.R.F. Effect of cyproterone acetate on cells of the pars distalis of the adenohypophysis in the Beagle Bitch. Cell Tissue Res. 183, 177–189 (1977). https://doi.org/10.1007/BF00226618

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