Summary
Genetic diversity and differentiation in indica and japonica groups of the cultivated rice (Oryza sativa L.) were studied by assaying DNA restriction fragment length polymorphisms of 12 indica and 14 japonica rice lines digested with three restriction endonucleases. A total of 49 probes were selected to represent the entire RFLP map at intervals of 20–30 cM. It was shown that 95 of the 145 possible probe/enzyme combinations, involving 43 probes and all three enzymes, detected restriction fragment length variation, and the degree of polymorphism varied greatly from one probe/enzyme combination to another. These results demonstrate that indica rice is genetically more diverse than japonica type. Significant differentiation between the two rice groups was detected by 33 probes representing 11 of the 12 rice chromosomes. It was deduced that the processes leading to differentiation involved a combination of molecular events that include base substitutions and insertion/deletions.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bowman KD, Hutcheson K, Odum EP, Shenton LR (1971) Comments on the distribution of indices of diversity. Stat Ecol 3:315–359
Cheng KS (1985) A statistical evaluation of the classification of rice cultivars into hsien and keng subspecies. Rice Genet Newsl 2:46–48
Cordesse F, Second G, Delseny M (1990) Ribosomal gene spacer length variability in cultivated and wild rice species. Theor Appl Genet 79:81–88
Feinberg AP, Vogelstein B (1983) A technique for radiolabelling DNA restriction fragment length polymorphisms to high specific activity. Anal Biochem 132:6–13
Gerlach WL, Bedbrook JR (1979) Cloning and characterization of ribosomal RNA genes from wheat and barley. Nucleic Acids Res 7:1869–1885
Glaszmann JC (1987) Isozymes and classification of Asian rice varieties. Theor Appl Genet 74:21–30
Ikehashi H, Araki H (1985) Genetics of F1 sterility in remote crosses of rice. In: IRRI (ed) Rice genetics. International Rice Genetics Symposium I Manila, Philippines, pp 119–130
Kempthorne O (1955) The randomization theory of experimental inference. J Am Stat Assoc 50:946–967
McCouch SR, Kodiert G, Yu ZH, Wang ZY, Khush GS, Coffman WR, Tanksley SD (1988) Molecular mapping of rice chromosomes. Theor Appl Genet 78:815–829
Morishima H, Oka HI (1981) Phylogenetic differentiation of cultivated rice. 21 Numerical evaluations of the indica-japonica differentiation. Jpn J Breed 31:402–413
Oka HI (1964) Considerations on the genetic basis of intervarietal sterility in Oryza sativa. In: IRRI (ed) Rice genetics and cytogenetics. Symposium on Rice Genetics and Cytogenetics Manila, Elsevier, Amsterdam, pp 159–174
Saghai Maroof MA, Soliman KM, Jorgensen RA, Allard RW (1984) Ribosomal DNA spacer-length polymorphisms in barley: Mendelian inheritance, chromosomal location, and population dynamics. Proc Natl Acad Sci USA 81:8014–8018
Second G (1982) Origin of the genic diversity of cultivated rice (Oryza spp): study of the polymorphism scored at 40 isozyme loci. Jpn J Genet 57:25–57
Zeng S, Yang X, Lu Z (1980) The heterosis of F1 hybrids between Oryza sativa subsp hsien and O. sativa subsp keng. Acta Agron Sin 6:193–202
Author information
Authors and Affiliations
Additional information
Communicated by Hu Han
Rights and permissions
About this article
Cite this article
Zhang, Q., Maroof, M.A.S., Lu, T.Y. et al. Genetic diversity and differentiation of indica and japonica rice detected by RFLP analysis. Theoret. Appl. Genetics 83, 495–499 (1992). https://doi.org/10.1007/BF00226539
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00226539