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Regulation of β-chain mRNA of ovine follicle-stimulating hormone by 17β-estradiol

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Summary

17β-Estradiol (E2) rapidly and reversibly decreases the synthesis of follicle-stimulating hormone (FSH) in primary dispersed cell cultures of ovine pituitaries. Similarly, E2 also causes a decrease in the messenger RNA for the beta subunit of ovine FSH (FSIIβ-mRNA) as measured in an in vitro translation assay. These results are consistent with the concept that E2 directly regulates either transcription of the FSHβ gene or processing of FSHβ-mRNA in sheep. Of additional interest is the observation that pituitary cultures from various species respond differently to E2 in terms of FSH synthesis. For example, E2 stimulates FSH synthesis in rat pituitary cultures, has no effect in similar rabbit cultures, and inhibits FSH synthesis in ovine cultures. Thus, a set of eukaryotic ‘mutants’ may exist to aid studies of the effect of E2 on FSH synthesis and secretion.

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References

  1. Notides, A. C., 1970. Endocrinology 87: 987–992.

    Google Scholar 

  2. Wise, P. M. and Payne, A. H., 1975. Mol. Cell Endocrinol. 3:385–395.

    Google Scholar 

  3. Tang, L. K. L. and Spies, H. G., 1975. Endocrinology 96: 349–356.

    Google Scholar 

  4. Labrie, F., Drouin, J., De Lean, A., Lagace, L., Ferland, L. and Beaulieu, M., 1977. Adv. Exp. Med. Biol. 87: 157–179.

    Google Scholar 

  5. Drouin, J., Lavoie, M. and Labrie, F., 1978. Endocrinology 102:358–361.

    Google Scholar 

  6. Steinberger, A. and Chowdhury, M., 1974. Endocr. Res. Commun. 1: 389–401.

    Google Scholar 

  7. Miller, W. L. and Wu, J., 1981. Endocrinology 108:673–679.

    Google Scholar 

  8. Ryan, R., Shupnik, M. A. and Gorski, J., 1979. Biochemistry 18: 2044–2048.

    Google Scholar 

  9. McKnight, G. S. and Palmiter, R. D., 1979. J. Biol. Chem. 254:9050–5058.

    Google Scholar 

  10. McKnight, G. S., Lee, D. C. and Palmiter, R. D., 1980. J. Biol. Chem. 255: 148–153.

    Google Scholar 

  11. Hayward, M. A., Mitchell, T. A. and Shapiro, D. J., 1980. J. Biol. Chem. 255: 11308–11312.

    Google Scholar 

  12. Kaplan, S. L., Grumbach, M. M. and Aubert, M. L., 1976. J. Clin. Endocrinol. Metab. 42: 995–998.

    Google Scholar 

  13. Landefeld, T. D., 1979. J. Biol. Chem. 254: 3685–3688.

    Google Scholar 

  14. Landefeld, T. D. and Kepa, J., 1979. Biochem. Biophys. Res. Commun. 90: 1111–1118.

    Google Scholar 

  15. Godine, J. E., Chin, W. W. and Habener, J. F., 1981. J. Biol. Chem. 256: 2475–2479.

    Google Scholar 

  16. Chin, W. W., Habener, J. F., Kieffer, J. D. and Maloof, F., 1978. J. Biol. Chem. 253: 7985–7988.

    Google Scholar 

  17. Giudice, L. C. and Weintraub, B. D., 1979. J. Biol. Chem. 254:12679–12683.

    Google Scholar 

  18. Huang, E. S. and Miller, W. L., 1980. Biol. Reprod. 23: 124–134.

    Google Scholar 

  19. Miller, W. L., Knight, M. M., Grimek, H. J. and Gorski, J., 1977. Endocrinology 100: 1306–1316.

    Google Scholar 

  20. Sherwood, O. D., Grimek, H. J. and McShan, W. H., 1970. J. Biol. Chem. 245: 2328–2336.

    Google Scholar 

  21. Sairam, M. R., 1979. Arch. Biochem. Biophys. 194: 71–78.

    Google Scholar 

  22. Alexander, D. C. and Miller, W. L., 1982. J. Biol. Chem. 257:2282–2286.

    Google Scholar 

  23. Alexander, D. C. and Miller, W. L., 1981. J. Biol. Chem. 256:12628–12631.

    Google Scholar 

  24. Grimek, H. J. and McShan, W. H., 1974. J. Biol. Chem. 249: 5725–5732.

    Google Scholar 

  25. Vician, L., Shupnik, M. A. and Gorski, J., 1979. Endocrinology 104:736–743.

    Google Scholar 

  26. Miller, W. L., Knight, M. M. and Gorski, J., 1977. Endocrinology 101: 1455–1460.

    Google Scholar 

  27. Miller, W. L. and Huang, E. S., 1981. Endocrinology 108: 96–102.

    Google Scholar 

  28. Scott, W. A. and Tomkins, G. M., 1975. In: Methods in Enzymology. (O'Malley, B. W. and Hardman, J. G., Eds.), Vol. XL, Part E, p. 278, New York: Academic Press.

    Google Scholar 

  29. Karsch, F. J., Foster, D. L., Legan, S. J., Ryan, K. D. and Peter, G. K., 1979. Endocrinology 105: 421–426.

    Google Scholar 

  30. Katzenellenbogen, B. S. and Gorski, J., 1975. In: Estrogen Actions on Synthesis of Macromolecules in Target Cells. (Litwack, G., ed.), p. 187, New York: Academic Press.

    Google Scholar 

  31. Pierce, J. G. and Parsons, T. F., 1981. Ann. Rev. Biochem. 50:465–495.

    Google Scholar 

  32. Godine, J. E., Chin, W. W. and Habener, J. F., 1980. J. Biol. Chem. 255: 8780–8783.

    Google Scholar 

  33. Sairam, M. R., Seiday, N. G. and Chretien, M., 1981. Biochem. J. 197: 541–552.

    Google Scholar 

  34. Schanbacher, B. D. and Ford, J. J., 1977. Endocrinology 100:387–393.

    Google Scholar 

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Author notes

  1. D. C. Alexander

    Present address: Plant Cell Research Institute, 6560 Trinity Court, 94566, Dublin, CA

  2. E. S. Huang

    Present address: Environmental Protection Agency, Health Effects Research Laboratory, Developmental Biology Division, Reproductive Toxicology Branch, 27711, Research Triangle Park, NC

Authors and Affiliations

  1. Department of Biochemistry, North Carolina State University, 27650-5050, Raleigh, NC, U.S.A.

    W. L. Miller, D. C. Alexander, J. C. Wu, E. S. Huang, G. K. Whitfield & S. H. Hall

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  1. W. L. Miller
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  2. D. C. Alexander
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  3. J. C. Wu
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  4. E. S. Huang
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  5. G. K. Whitfield
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  6. S. H. Hall
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Additional information

Contribution from the Department of Biochemistry, School of Agriculture and Life Sciences and School of Physical and Mathematical Sciences. Paper No. 8529 of the Journal Series of the North Carolina Agricultural Research Service, Raleigh, North Carolina 27650-5050.

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Miller, W.L., Alexander, D.C., Wu, J.C. et al. Regulation of β-chain mRNA of ovine follicle-stimulating hormone by 17β-estradiol. Mol Cell Biochem 53, 187–195 (1983). https://doi.org/10.1007/BF00225253

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  • DOI: https://doi.org/10.1007/BF00225253

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