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Changes in pituitary gonadotropin subunits and hypothalamic Kiss-1 gene expression by administration of sex steroids in ovary-intact female rats

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Abstract

Objective

We examined how the sex steroids influence the synthesis of gonadotropins.

Materials and Methods

The effects of sex steroids estradiol (E2), progesterone (P4), and dihydrotestosterone (DHT) in pituitary gonadotroph cell model (LβT2 cells) in vitro and ovary-intact rats in vivo were examined. The effects of sex steroids on Kiss1 gene expression in the hypothalamus were also examined in ovary-intact rats.

Results

In LβT2 cells, E2 increased common glycoprotein alpha (Cga) and luteinizing hormone beta (Lhb) subunit promoter activity as well as their mRNA expression. Although gonadotropin subunit promoter activity was not modulated by P4, Cga and Lhb mRNA expression was increased by P4. DHT inhibited Cga and Lhb mRNA expression with a concomitant decrease in their promoter activity. During the 2-week administration of exogenous E2 to ovary-intact rats, the estrous cycle determined by vaginal smears was disrupted. P4 or DHT administration completely eliminated the estrous cycle. Protein expression of all three gonadotropin subunits within the pituitary gland was inhibited by E2 or P4 treatment in vivo; however, DHT reduced Cga expression but did not modulate Lhb or follicle-stimulating hormone beta subunit expression. E2 administration significantly repressed Kiss1 mRNA expression in a posterior hypothalamic region that included the arcuate nucleus. P4 and DHT did not modulate Kiss1 mRNA expression in this region. In contrast, P4 administration significantly inhibited Kiss1 mRNA expression in the anterior region of the hypothalamus that included the anteroventral periventricular nucleus. The expression of gonadotropin-releasing hormone (Gnrh) mRNA in the anterior hypothalamic region, where the preoptic area is located, appeared to be decreased by treatment with E2 and P4.

Conclusion

Our findings suggest that sex steroids have different effects in the hypothalamus and pituitary gland.

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Data Availability

The datasets used and/or analyzed during this study are available from the corresponding authors on reasonable request.

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Acknowledgements

The manuscript was edited by ThinkSCIENCE, Inc.

Funding

This work was supported in part by Grants-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology of Japan (to H.K. and A.O.).

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Authors and Affiliations

  1. Department of Obstetrics and Gynecology, Shimane University School of Medicine, Izumo, 693-8501, Japan

    Susdiaman S. Yacca, Haruhiko Kanasaki, Tuvshintugs Tumurbaatar, Zhuoma Cairang, Aki Oride, Hiroe Okada & Satoru Kyo

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  1. Susdiaman S. Yacca
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Correspondence to Haruhiko Kanasaki.

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Yacca, S.S., Kanasaki, H., Tumurbaatar, T. et al. Changes in pituitary gonadotropin subunits and hypothalamic Kiss-1 gene expression by administration of sex steroids in ovary-intact female rats. Endocrine 83, 733–746 (2024). https://doi.org/10.1007/s12020-023-03596-0

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