Abstract
Genome-specific DNA markers are of great value in many applications. Recent work on different plants and animal species indicated that PCR- (polymerase chain reaction) based genetic marker systems using specific primers are highly genome-specific. To test the genome specificity of sequence-tagged-sites (STSs) as genetic markers in Stylosanthes, 20 pairs of primers were generated. Fifteen were from randomly selected single-copy Pstl genomic clones, and the other five were from two known gene sequences. These primer pairs were analysed against a set of 24 genotypes representing 12 different Stylosanthes species. Thirteen of these primer pairs amplified successfully. Overall, there was a low level of genome specificity, suggesting a low degree of genomic divergence within this group of Stylosanthes species. Of the 312 entries (24 genotypes by 13 primer pairs), PCR amplifications were unsuccessful (little or no products) in only 16 cases. The number of banding patterns detected by each of these primer pairs varied from 2 to 12 with an average pair-wise polymorphism of 44.3%. The level of intraspecific variation detected on normal agarose gels was only 3.8%. Further evidence that diploid S. hamata and diploid S. humilis are progenitors of tetraploid S. hamata and that S. viscosa is a progenitor of S. scabra, was obtained.
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References
Cameron DF (1967) Chromosome number and morphology of some introduced Stylosanthes species. Aust J Agric Res 18:375–379
Curtis MD, Cameron DF, Manners JM (1995) Molecular evidence that diploid Stylosanthes humilis and diploid Stylosanthes hamata are progenitors of allotetraploid Stylosanthes hamata. Genome 38:344–348
Devos KM, Bryan GJ, Collins AJ, Stephenson P, Gale MD (1995) Application of two microsatellite sequences in wheat storage proteins as molecular markers. Theor Appl Genet 90:247–252
Kirkbride JH, de Kirkbride CG (1985) Typification of Stylosanthes (Leguminosae) and its sections. Taxon 36:455–458
Liu CJ, Musial JM (1995) Restriction fragment length polymorphism detected by cDNA and genomic DNA clones in Stylosanthes. Theor Appl Genet 91:1210–1213
Money TA, Liu CJ, Gale MD (1994) Conversion of RFLP markers for downy mildew resistance in pearl millet to sequence-taggedsites. In: Witcombe RJ, Duncan RR (eds) Use of molecular markers in sorghum and pearl millet breeding for developing countries. Proc ODA Plant Sci Res Conf, Overseas Development Administration, London, pp 65–68
Smith FW, Ealing PM, Hawkesford MJ, Clarkson DT (1995) Plant members of a newly defined family of sulfate transporters reveal functional sub-types. Proc Natl Acad Sci USA 92:9373–9377
Stace HM, Cameron DF (1984) Cytogenetics and the evolution of Stylosanthes. In: Stace HM, Edye LA (eds) The biology and agronomy of Stylosanthes. Academic Press, Sydney, pp 49–72
Tragoonrung S, Kanazin V, Hayes PM, Blake TK (1992) Sequence-tagged-site-facilitated PCR for barley genome mapping. Theor Appl Genet 84:1002–1008
Wolff K, Peters-Van Rijn J, Hofstra H (1994) RFLP analysis in chrysanthemum. I. Probe and primer development. Theor Appl Genet 88:472–478
Wu DY, Ugozzoli L, Pal BK, Wallace RB (1989) Allele-specific enzymatic amplification of beta globin genomic DNA for diagnosis of sickle cell anemia. Proc Natl Acad Sci USA 86:2757–2760
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Communicated by G. Wenzel
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Liu, C.J., Musial, J.M. & Smith, F.W. Evidence for a low level of genomic specificity of sequence-tagged-sites in Stylosanthes . Theoret. Appl. Genetics 93, 864–868 (1996). https://doi.org/10.1007/BF00224087
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DOI: https://doi.org/10.1007/BF00224087