Summary
Medicago sativa L. cv Regen S is heteroplasmic for chloroplast DNA (cpDNA). Previous analyses of regenerated plants have shown a predominance of one of the cpDNAs which we have designated type A (the other we have designated type B). Studies of the replication of the two cpDNAs in tissue culture were carried out using leaflet expiants with defined cpDNA types and a distinguishing probe. The explants obtained showed a bias toward type A cpDNA during tissue culture. The data suggest that chloroplasts with different DNAs in a common nuclear background can multiply at different rates.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Chiu W-L, Sears BB (1992) Electron microscope localization of replication origins in Oenothera chloroplast DNA. Mol Gen Genet 232:33–39
Chiu W-L, Stubbe W, Sears BB (1988) Plastid inheritance in Oenothera: organelle genome modifies the extent of biparental plastid transmission. Curr Genet 13:181–189
Corriveau JL, Coleman AW (1988) Rapid screening method to detect potential biparental inheritance of plastid DNA and results for over 200 angiosperm species. Am J Bot 75:1443–1458
Dalton CC, Iqbal K, Turner DA (1983) Iron phosphate precipitation in Murashige and Skoog media. Physiol Plant 57:472–476
Dorfel P, Weihe A, Knosche R, Borner T (1989) Mitochondrial DNA of Chenopodium album (L.): a comparison of leaves and suspension cultures. Curr Genet 16:375–380
Gamborg OL, Miller RA, Ojima K (1968) Nutrient requirements of suspension cultures of soybean root cells. Exp Cell Res 50; 151–158
Haccius B (1978) Question of unicellular origin of non-zygotic embryos in callus cultures. Phytomorphology 28:74–81
Hartmann CY, Henry Y, De Buyser J, Lejeune B, Quetier F, Rode A (1989) A comparative study of the mitochondrial DNA organization in nonembryogenic and embryogenic somatic tissue cultures of wheat: evidence for a differential variability. Giorn Bot Ital 123:123–312
Johnson LB, Palmer JD (1989) Heteroplasmy of chloroplast DNA in Medicago. Plant Mol Biol 12:3–11
Kao KN, Wetter LR (1977) Advances in techniques of plant protoplast fusion and culture of heterokaryocytes. In: Brinkley BR, Porter K (eds) International Cell Biology 1976–1977, New York. Rockefeller University Press, New York, pp 216–224
Lee DJ, Blake TK, Smith SE (1988) Biparental inheritance of chloroplast DNA and the existence of heteroplasmic cells in alfalfa. Theor Appl Genet 76:545–549
Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York
Masoud SA, Johnson LB, Sorensen EL (1990) High transmission of paternal plastid DNA in alfalfa plants demonstrated by restriction fragment polymorphic analysis. Theor Appl Genet 79:49–55
Mettler IJ (1987) A simple and rapid method for minipreparation of DNA from tissue cultured plant cells. Plant Mol Biol Rep 5:346–349
Murashige T, Skoog A (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497
Ozias-Akins P, Tabaeizadeh Z, Pring DR, Vasil IK (1988) Preferential amplification of mitochondrial DNA fragments in somatic hybrids of the Gramineae. Curr Genet 13:241–245
Palmer JD, Osorio B, Aldrich J, Thompson WF (1987) Chloroplast DNA evolution among legumes: loss of a large inverted repeat occurred prior to other sequence rearrangements. Curr Genet 11:275–286
Reed KC, Mann DA (1985) Rapid transfer of DNA from agarose gels to nylon membranes. Nucleic Acids Res 13:7207–7221
Rose RJ, Johnson LB, Kemble RJ (1986) Restriction endonuclease studies on the chloroplast and mitochondrial DNAs of alfalfa (Medicago sativa L.) protoclones. Plant Mol Biol 6:331–338
Rose RJ, Thomas MR, Fitter JT (1990) The transfer of cytoplasmic and nuclear genomes by somatic hybridisation. Aust J Plant Physiol 17:303–321
Schumann CM, Hancock JF (1989) Paternal inheritance of plastids in Medicago sativa. Theor Appl Genet 78:863–866
Shirzadegan M, Palmer JD, Christey M, Earle Ed (1991) Patterns of mitochondrial instability in Brassica campestris cultured cells. Plant Mol Biol 16:21–37
Smith SE, Bingham ET, Fulton RW (1986) Transmission of chlorophyll deficiencies in Medicago sativa. J Hered 77:35–38
Thomas MR, Johnson LB, White FF (1990) Selection of interspecific somatic hybrids of Medicago by using Agrobacterium transformed tissues. Plant Sci 69:189–198
Author information
Authors and Affiliations
Additional information
Communicated by R. Hagemann
Rights and permissions
About this article
Cite this article
Fitter, J.T., Rose, R.J. Investigation of chloroplast DNA heteroplasmy in Medicago sativa L. using cultured tissue. Theoret. Appl. Genetics 86, 65–70 (1993). https://doi.org/10.1007/BF00223809
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00223809