Abstract
Twelve Japanese rice cultivars were converted to CMS by asymmetric protoplast fusion with MTC-5A, the cytoplasm of which was derived from an indica rice, Chinsurah Boro II. With the exception of the cybrids that had a nucleus from Hoshiyutaka, most of these cybrid plants were sterile. The unique sequence downstream from the mitochondrial atp6 of MTC-5A was specifically amplified in the sterile cybrid plants by PCR. All progenies of the cybrid plants carrying this unique sequence were sterile. On the other hand, in some of the sterile cybrid plants in which the unique sequence was not amplified by PCR, fertility was recovered in their progenies. Somaclonal mutation may have caused sterility in these cybrids. Only the cybrid plants that had the unique sequence detected by PCR were CMS. Thus, the CMS plants can be selected rapidly and easily by PCR, at an early stage of plant regeneration. Soon after transplanting the regenerated plants to a green house, fertile cybrids and sterile cybrids produced by somaclonal mutation can be removed. These findings also show that the unique region downstream from atp6 is tightly linked with the CMS phenotype.
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References
Akagi H, Sakamoto M, Negishi T, Fujimura T (1989) Construction of rice cybrid plants. Mol Gen Genet 21:501–506
Akagi H, Sakamoto M, Shinjyo C, Shimada H, Fujimura T (1994) A unique sequence located downstream from the rice mitochondrial atp6 may cause male sterility. Curr Genet 25:52–58
Dewey RE, Levings III CS, Timothy DH (1986) Novel recombinations in the maize mitochondrial genome produce a unique transcriptional unit in the Texas male-sterile cytoplasm. Cell 44:439–449
Edwards K, Johnstone C, Thompson C (1991) A simple and rapid method for the preparation of plant genome DNA for PCR analysis. Nucleic Acids Res 19:1349
Fujimura T, Sakurai M, Akagi H, Negishi T, Hirose A (1985) Regeneration of rice plants from protoplasts. Plant Tissue Cult Lett 2:74–75
Handa H, Nakajima K (1992) Different organization and altered transcription of the mitochondrial atp6 gene in the male-sterile cytoplasm of rapeseed (Brassica napus L.). Curr Genet 21:153–159
Iwabuchi M, Kyouzuka J, Shimamoto K (1993) Processing followed by complete editing of an altered mitochondrial atp6 RNA restores fertility of cytoplasmic male-sterile rice. EMBO J 12:1437–1446
Katsuo K, Mizushima U (1958) Studies on the cytoplasmic difference among rice varieties, Oryza saliva L. I. On the fertility of hybrids obtained reciprocally between cultivated and wild varieties. Japan J Breed 8:1–5
Li Z, Zho Y (1988) Rice male-sterile cytoplasm and fertitliy restoration. In: Hybrid rice. Int Rice Res Inst, Manila, Philippines, pp 85–102
Newton KJ (1988) Plant mitochondrial genomes: organization, expression and variation. Annu Rev Plant Physiol Plant Mol Biol 39:503–532
Shinjyo C (1972) Distributions of male sterility inducing cytoplasms and fertility restoring genes in rice. II. Varieties introduced from sixteen countries. Japan J Breed 22:329–333
Singh M, Brown GG (1991) Suppression of cytoplasmic male sterility by nuclear genes alters expression of a novel mitochondrial gene region. Plant Cell 3:1349–1362
Young EG, Hanson MR (1987) A fused mitochondrial gene associated with cytoplasmic male sterility is developmentally regulated. Cell 50:41–49
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Communicated by D. R. Pring
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Akagi, H., Nakamura, A., Sawada, R. et al. Genetic diagnosis of cytoplasmic male sterile cybrid plants of rice. Theoret. Appl. Genetics 90, 948–951 (1995). https://doi.org/10.1007/BF00222907
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DOI: https://doi.org/10.1007/BF00222907