Summary
In the pituitary of the trout, the corticotropic and melanotropic cells display a strong immunocytological reaction with α-endorphin antiserum. This reaction persists even when a-endorphin antisera treated with β-1-24 ACTH or α-MSH are used. In the absence of pharmacological tests on the endorphic potencies of the compounds involved in the immunoreaction, it is not yet clear whether this reaction is due to the presence of an α-endorphin-like peptide or simply an immunologically related peptide without the properties of endorphin. However, the presence of such peptides in the fish pituitary is interesting from the comparative point of view.
Similar content being viewed by others
References
Bachelot, I., Wolfsen, A.R., Odell, W.D.: Pituitary and plasma lipotropins: demonstration of the artifactual nature of β-MSH. J. clin. Endocr. 44, 939–946 (1977)
Ball, J.N., Baker, B.I.: The pituitary gland: Anatomy and histophysiology. In: Fish physiology (W.S. Hoar and D.J. Randall, eds.), Vol. 2, pp. 1–110. New York: Academic press. 1969
Bloom, F., Battenberg, E., Rossier, S., Ling, N., Leppaluoto, J., Vargo, T., Guillemin, R.: Endorphins are located in the intermediate and anterior lobes of the pituitary gland, not in the neurohypophysis. Life Sci. 20, 43–48 (1977)
Chrétien, M., Benjannet, S., Dragon, N., Seidah, N.G., Lis, M.: Isolation of peptides with opiate activity from sheep and human pituitaries: relationship to beta-lipotropin. Biochem. biophys. Res. Commun. 72, 472–478 (1976)
Cox, B.M., Opheim, K.E.: Teschenmacher, H., Goldstein, A.: A peptide-like substance from pituitary that acts like morphine 2) Purification and properties. Life Sci. 16, 1777–1782 (1975)
Crine, P., Benjannet, S., Seidah, N.G., Lis, M., Chrétien, M.: In vitro biosynthesis of β-endorphin in pituitary glands. Proc. nat. Acad. Sci. (Wash.) 74, 1403–1406 (1977)
Dessy, C., Herlant, M.: Localisation comparée de l'immunserum antilipotropine, anticorticotropine et antimelanotropine au niveau de l'hypophyse de porc. C.R. Acad. Sci. (Paris) 278D, 1923–1926 (1974)
Dessy, C., Herlant, M., Chrétien, M.: Detection par immuno-fluorescence des cellules synthetisant la lipotropine. C.R. Acad. Sci. (Paris) 276, D 335–338 (1973)
Doerr-Schott, J., Dubois, M.P.: Identification par immunofluorescence des cellules corticotropes et mélanotropes de l'hypophyse des amphibiens. Z. Zellforsch. 132, 323–331 (1972)
Dubois, M.P.: Localisation cytologique par immunofluorescence des secrétions corticotropes, a et β mélanotropes au niveau de l'adénohypophyse des bovins, ovins et porcins. Z. Zellforsch. 125, 200–209 (1972a)
Dubois, M.P.: Nouvelles données sur la localisation au niveau de l'adénohypophyse des hormones polypeptidiques: ACTH, MSH, LPH. Lille med. 17, 1391–1394; engl. abstr. 1418 (1972b)
Dubois, M.P.: Observation indicated in Bloom et al., 1977
Follénius, E., Dubois, M.P.: Etude immunocytologique des cellules corticotropes de plusieurs espèces de poissons téléostéens: Gasterosteus aculeatus L., Carassius auratus L., Lebistes reticulatus P., Salmo irideus Gibbs et Perca fluviatilis L. Gen. comp. Endocr. 28, 339–349 (1976)
Guillemin, R., Ling, N., Burgus, R.: Endorphines peptides d'origine hypothalamique et neurohypophysaire à activité morphinomimétique. Isolement et structure moléculaire de l'α endorphine. C.R. Acad. Sci. (Paris) 282D, 783–785 (1976)
Guillemin, R., Vargo, T., Rossier, J., Minick, S., Ling, N., Rivier, C., Vale, W., Bloom, F.: β-endorphins and adrenocorticotropin are secreted concomitantly by the pituitary gland. Science 197, 1367–1369 (1977)
Li, C.H., Barnafi, L., Chrétien, M., Chung, D.: Isolation and aminoacid sequence of βLPH from sheep pituitary glands. Nature (Lond.) 208, 1093–1094 (1965)
Lowry, P.J., Scott, A.P.: Structural relationship and biosynthesis of corticotrophin, lipotrophin and melanotrophin. In: Melanocyte stimulating hormone: Control chemistry and effects (F.J.H. Tilders, D.F. Swaab and T.B. van Wiersma Greidanus, eds.). Frontiers in hormone research, No. 4, pp. 11–17. Basel-New York: Karger (1977)
Mains, R.E., Eipper, B.A., Ling, N.: Common precursor to corticotropins and endorphins. Proc. nat. Acad. Sci. (Wash.) 74, 3014–3018 (1977)
Malo-Michéle, M., Bugnon, Cl., Fellmann, D.: Etude cyto-immunologique des cellules corticotropes et cortico-mélanotropes de l'adénohypophyse de la Saupe, Boops, Salpa L. (Téléostéen marin) dans différentes conditions (Variations de salinité et couleur de fond) C.R. Acad. Sci. (Paris) 283 D, 643–646 (1976)
Moon, H.D., Li, C.H., Jennings, B.M.: Immunohistochemial and histochemical studies of pituitary β-lipotrophs. Anat. Rec. 175, 529–538 (1973)
Moriarty, G.G., Halmi, N.S.: Adrenocorticotropin production by the intermediate lobe of the rat pituitary: an electron microscopic-immunohistochemical study. Z. Zellforsch. 132, 1–14 (1972)
Naik, D.U.: Immunohistochemical localization of adrenocorticotropin and melanocyte stimulating hormone in pars intermedia of rat hypophysis. Z. Zellforsch. 142, 289–304 (1973)
Olivereau, M., Bugnon, Cl., Fellmann, D.: Localisation cyto-immunologique de αMSH et d'ACTH dans les cellules hypophysaires colorables avec l'hématoxyline au plomb (HPb) chez l'Anguille. C.R. Acad. Sci. (Paris) 283D, 1321–1323 (1976a)
Olivereau, M., Bugnon, Cl., Fellmann, D.: Identification cyto-immunologique de deux catégories cellulaires dans le lobe intermédiaire de l'hypophyse des Salmonidés: présence d'ACTH et d'αMSH. C.R. Sci. (Paris) 283D, 1441–1443 (1976b)
Pelletier, G., Leclerc, R., Labrie, F., Cote, J., Chrétien, M., Lis, M.: Immunohistochemical localization of β-lipotropic hormone in the pituitary gland. Endocrinology 100, 770–776 (1977)
Queen, G., Pinsky, C., LaBella, F.: Subcellular localization of endorphine activity in bovine pituitary and brain. Biochem. biophys. Res. Commun. 72, 1021–1027 (1976)
Romain, R., Bugnon, C., Dessy, C., Fellmann, D.: Etude cytoimmunologique des cellules hématoxyline au plomb-positives de l'adénohypophyse de la tanche. (Tinca tinca L.)
Rossier, J., Bloom, F., Battenberg, E., Vargo, T., Ling, N., Guillemin, R.: Identification of α-endorphin and β-endorphin in rat brain: Radioimmunoassay and immunocytochemical localization. VIth Int. Meeting of the Int. Soc. for Neurochemistry Copenhagen 1977, p. 266
Roux, M., Dubois, M.P.: Immunohistochemical study of the pars intermedia of the mouse pituitary in different experimental conditions. Experientia (Basel) 32, 657–658 (1976)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Follénius, E., Dubois, M.P. Immunocytological detection and localization of a peptide reacting with an α-endorphin antiserum in the corticotropic and melanotropic cells of the trout pituitary (Salmo irideus Gibb). Cell Tissue Res. 188, 273–283 (1978). https://doi.org/10.1007/BF00222637
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00222637