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Coexpression of opsin- and VIP-like-immunoreactivity in CSF-contacting neurons of the avian brain

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Summary

Cerebrospinal fluid-contacting (CSF) cells in both the septal and the tuberal areas in the brain of the ring dove are labeled by RET-P1, a monoclonal antibody to opsin that reacts with inner and outer segment membranes of rod photoreceptors in a variety of vertebrates. Immunoblot analysis of proteins from diverse brain regions, however, revealed bands of anti-RET-P1 immunoreactivity that did not correspond to opsin. Binding of RET-P1 to opsin-containing membranes, was not inhibited by membranes rich in muscarinic and β-adrenergic receptor proteins (red blood cells, heart, lung) taken from doves. RET-P1-immunoreactive CSF-contacting cells emit a dendritic process that penetrates the ependyma and ends in a knob-like terminal suspended in the ventricle. These cells also possess other processes that penetrate more or less deeply into the neuropil. Additionally, a band of labeled fibers occurs in the external layer of the median eminence. A double-label technique demonstrated that RET-P1-positive cells coexpress VIP-like immunoreactivity. VIP-positive cells in other brain areas are not RET-P1-positive.

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Authors and Affiliations

  1. Department of Psychology, Barnard College of Columbia University, New York, USA

    Rae Silver & P. Horvath

  2. Department of Ophthalmology, New York University, New York, USA

    P. Witkovsky & V. Alones

  3. Rockefeller University, New York, USA

    C. J. Barnstable

  4. Department of Anatomy and Cell Biology, University of Cincinnati, Cincinnati, Ohio, USA

    M. N. Lehman

Authors
  1. Rae Silver
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  2. P. Witkovsky
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  3. P. Horvath
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  4. V. Alones
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  5. C. J. Barnstable
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  6. M. N. Lehman
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Silver, R., Witkovsky, P., Horvath, P. et al. Coexpression of opsin- and VIP-like-immunoreactivity in CSF-contacting neurons of the avian brain. Cell Tissue Res. 253, 189–198 (1988). https://doi.org/10.1007/BF00221754

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  • DOI: https://doi.org/10.1007/BF00221754

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