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Maturation of adult rat peritoneal and mesenteric mast cells

A morphological and histofluorescence study

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Summary

Repopulation and maturation of rat mesenteric and peritoneal mast cells were studied after mast cell depletion by intraperitoneal injection of distilled water. Immature mast cells were first identified in the mesentery and peritoneal fluid 5 and 6 days, respectively, after water injection. The most immature mast cells that could be identified contained a few orthochromatic granules. Upon maturation, the granules became metachromatic and increased in size and number. Heparin, revealed by toluidine blue staining and berberine sulfate fluorescence, appeared simultaneously with orthophthaldialdehyde (OPT)-induced histamine fluorescence. Paraformaldehyde-induced serotonin fluorescence appeared somewhat later. Repopulation of mesentery and peritoneal fluid by mast cells seemed to be independent of each other and to occur from undifferentiated precursor cells.

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Author notes

  1. Valmor O. Mendonça

    Present address: Departamento de Ciências Morfológicas, Fundação Universidade do Rio Grande, Brasil

  2. Maria Celia Jamur

    Present address: Departamento de Ciências Morfológicas, Universidade Federal do Parana. Fellow of CAPES, Brasil

Authors and Affiliations

  1. Departamento de Morfologia, Faculdade de Medicina, Ribeirão Preto, São Paulo, Brasil

    Valmor O. Mendonça, Ithamar Vugman & Maria Celia Jamur

Authors
  1. Valmor O. Mendonça
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  2. Ithamar Vugman
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  3. Maria Celia Jamur
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Mendonça, V.O., Vugman, I. & Jamur, M.C. Maturation of adult rat peritoneal and mesenteric mast cells. Cell Tissue Res. 243, 635–639 (1986). https://doi.org/10.1007/BF00218072

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  • DOI: https://doi.org/10.1007/BF00218072

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