Abstract
Mouse bone marrow-derived mast cells (mBMMCs) are an invaluable tool for the study of mast cell function as they represent a primary source of mature mast cells. They can be sourced from wild-type, knockout, and transgenic mice and are used to repopulate mast cell-deficient mice. This method describes the isolation of mast cell hematopoietic progenitors from the bone marrow of mouse femurs and their subsequent culture in an IL-3-rich culture medium. After 4 weeks in culture, mBMMCs are obtained in high number and are of high purity. Assessment of their granularity by toluidine staining and IgE receptor expression by flow cytometry is also described. These cells are a useful tool in the determination of in vitro and in vivo mast cell function in innate and adaptive immunity.
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Acknowledgments
Financial support for work in the authors’ laboratories was provided by the Faculty of Medicine, University of Southampton, and the Asthma, Allergy, and Inflammation Research charity.
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Swindle, E.J. (2020). Generation of Mast Cells from Murine Stem Cell Progenitors. In: Gibbs, B.F., Falcone, F.H. (eds) Basophils and Mast Cells. Methods in Molecular Biology, vol 2163. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0696-4_7
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DOI: https://doi.org/10.1007/978-1-0716-0696-4_7
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-0695-7
Online ISBN: 978-1-0716-0696-4
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