Summary
X-ray microanalysis of frozen-hydrated specimens of the cicada filter chamber shows that there are concentration changes in K+ and Cl- in the various compartments of the filter chamber from feeding to non-feeding states. These changes are consistent with an osmotic transfer of water between the anterior midgut and the posterior midgut and Malpighian tubules. K+ and Cl- account for a large proportion of the osmotically active solutes in these compartments, and it is suggested that the osmotic gradients are generated by secretion of K+ and Cl- into the posterior midgut and Malpighian tubule lumina. Analysis of freeze-substituted sections of filter chamber supports the analysis of frozen-hydrated specimens and indicates that Na+ may also be secreted into the posterior midgut and the Malpighian tubules. The system functions as a water-shunting complex.
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References
Berridge MJ (1970) A structural analysis of intestinal absorption. In: Neville AC (ed) Insect ultrastructure. Blackwell Scientific Publications, Oxford, p 135–151
Blankemeyer JT (1981) Active transport of potassium by insect midgut. Fed Proc 40:2412–2416
Cheung WWK, Marshall AT (1973a) Water and ion regulation in cicadas in relation to xylem feeding. J Insect Physiol 19:1801–1816
Cheung WWK, Marshall AT (1973b) Studies on water and ion transport in homopteran insects: ultrastructure and cytochemistry of the cicadoid and cercopoid midgut. Tissue and Cell 5:651–669
Diamond JM (1978) Solute-linked water transport in epithelia. In: Hoffman JF (ed) Membrane transport processes, Vol 1. Raven Press, New York, p 257–276
Dow JAT (1981a) Localization and characterization of water uptake from the midgut of the locust, Schistocerca gregaria. J Exp Biol 93:269–281
Dow JAT (1981b) Countercurrent flows, water movements and nutrient absorption in the locust midgut. J Insect Physiol 27:579–585
Dow JAT (1981c) Ion and water transport in locust alimentary canal: evidence from in vivo electrochemical gradients. J Exp Biol 93:167–179
Goodchild AJP (1966) Evolution of the alimentary canal in the hemiptera. Biol Rev 41:97–140
Gouranton J (1968) Ultrastructures en rapport avec un transit d'eau. Etude de la chambre filtrante de Cicadella viridus L. (Homoptera, Jassidae). J Microsc 7:559–574
Grantham JJ (1971) Mode of water transport in mammalian collecting tubules. Fed Proc 30:14–21
Kaissling B, Kriz W (1979) Structural analysis of the rabbit kidney. Springer, Berlin
Lindsay KL, Marshall AT (1980) Ultrastructure of the filter chamber complex in the alimentary canal of Eurymela distincta Signoret (Homoptera, Eurymelidae). Int J Insect Morphol Embryol 9:179–198
Lindsay KL, Marshall AT (1981) The osmoregulatory role of the filter chamber in relation to phloem-feeding in Eurymela distincta (Cicadelloidea, Homoptera). Physiol Entomol 6:413–419
Marshall AT (1977a) Electron probe X-ray microanalysis of frozen-hydrated biological specimens. Microscopica Acta 79:254–260
Marshall AT (1977b) Iso-atomic droplets as models for the investigation of parameters affecting X-ray microanalysis of biological specimens. Micron 8:193–200
Marshall AT (1980a) Quantitative X-ray microanalysis of frozen-hydrated bulk biological specimens. Scanning Electron Microsc 1980(2):335–348
Marshall AT (1980b) Freeze-substitution as a preparation technique for biological X-ray microanalysis. Scanning Electron Microsc 1980(2): 395–408
Marshall AT (1982) Application of φ(ϱz) curves and a windowless detector to the quantitative analysis of frozen-hydrated bulk biological specimens. Scanning Electron Microsc 1982(1): 243–260
Marshall AT, Cheung WWK (1973) Studies on water and ion transport in homopteran insects: ultrastructure and cytochemistry of the cicadoid and cercopoid hindgut. Tissue and Cell 5:671–678
Marshall AT, Cheung WWK (1974) Studies on ion and water transport in homopteran insects: ultrastructure and cytochemistry of the cicadoid and cercopoid Malpighian tubules and filter chamber. Tissue and Cell 6:153–171
Pallaghy CK (1973) Electron probe microanalysis of potassium and chloride in freeze-substituted leaf sections of Zea mays. Aust J Biol Sci 26:1015–1034
Peaker M (1979) Control mechanisms in birds. In: Gilles R (ed) Mechanisms of osmoregulation in animals. John Wiley and Sons, Chichester, p 323–348
Russ JC (1974) The direct element ratio model for quantitative analysis of thin sections. In: Hall T, Echlin P, Kaufmann R (eds) Microprobe analysis as applied to cells and tissues. Academic Press, London, p 269–276
Schmidt-Nielsen B (1971) Comparative aspects of transport of hypertonic, isotonic and hypotonic solutions by epithelial membranes. Fed Proc 30:3–5
Sokal RR, Rohlf FJ (1969) Biometry: the principles and practice of statistics in biological research. WH Freeman & Co., San Francisco
Stephenson JL (1978) Countercurrent transport in the kidney. Ann Rev Biophys Bioeng 7:315–339
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Marshall, A.T. X-ray microanalysis of the filter chamber of the cicada, Cyclochila australasiae Don.. Cell Tissue Res. 231, 215–227 (1983). https://doi.org/10.1007/BF00215788
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DOI: https://doi.org/10.1007/BF00215788