Summary
Specific binding sites for estrogen, testosterone, and progesterone have been demonstrated in the pituitary gland of mature male and female platyfish (Xiphophorus maculatus). With a histochemical procedure, fluorescent-steroid-hormone conjugates were localized in the cytoplasm and nucleus of the gonadotrops of the caudal pars distalis (CPD) and in cells of the pars intermedia (PI) previously demonstrated to contain immunoreactive gonadotropin. The specificity of the response was confirmed by means of competitive binding analyses and by using fluoresceinated BSA not linked to steroids. The physiological significance of steroid binding in the PI, as well as in the CPD, is discussed in the light of other recent studies on the pituitary gland of the platyfish.
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References
Chamness GC, Mercer WD, McGuire WL (1980) Are histochemical methods for estrogen receptor valid? J Histochem Cytochem 8:792–798
Clark JH, Eriksson HA, Hardin JW (1976) Uterine receptor-estradiol complexes and their interaction with nuclear binding sites. J Biochem 7:1039–1043
Gaetjens E, Pertschuk LP (1980) Synthesis of fluorescein labeled steroid hormone-albumin conjugates for the fluorescent histochemical detection of hormone receptors. J Steroid Biochem 13:1001–1003
Gorski J, Gannon F (1976) Current models of steroid hormone action: a critique. Ann Rev Physiol 38:425–450
Herbert DC, Weaker FJ, Sheridan PJ (1981) Localization of 3H-dihydotestosterone in the pituitary of the rhesus monkey. Cell Tissue Res 215:499–504
Kallman KD, Borkoski V (1978) A sex-linked gene controlling the onset of sexual maturity in female and male platyfish (Xiphophorus maculatus), fecundity in females and adult size in males. Genetics 89:79–119
Kallman KD, Schreibman MP (1973) A sex-linked gene controlling gonadotrop differentiation and its significance in determining the age of sexual maturation and size of the platyfish, Xiphophorus maculatus. Gen Comp Endocrinol 21:287–304
Kallman KD, Schreibman MP, Borkoski V (1973) Genetic control of gonadtrop differentiation in the platyfish, Xiphophorus maculatus (Poeciliidae). Science 181:678–680
Kim YS, Stumpf WE, Sar M, Martinez-Vargas MC (1978) Estrogen and androgen target cells in the brain of fishes, reptiles and birds: Phylogeny and ontogeny. Am Zool 18:425–433
Kim YS, Sar M, Stumpf WE (1979) Estrogen target cells in the pituitary of platyfish, Xiphophorus maculatus. Cell Tissue Res 198:435–440
Margolis-Kazan H, Schreibman MP (1981) Cross-reactivity between human and fish pituitary hormones as demonstrated by immunocytochemistry. Cell Tissue Res 221:257–267
Margolis-Kazan H, Peute J, Schreibman MP, Halpern LR (1981) Ultrastructural localization of gonadotropin and luteinizing hormone releasing hormone in the pituitary gland of a teleost fish (the platyfish). J Exp Zool 215:99–102
Markaverich BM, Williams MM, Upchurch S, Clark JH (1981) Heterogeneity of nuclear estrogen-binding sites of the rat uterus: a simple method for the quantitation of type I and type II sites by (3H) estradiol exchange. Endocrinology 109:62–69
Morrell JI, Pfaff DW (1978) A neuroendocrine approach to brain function: localization of sex steroid concentrating cells in vertebrate brains. Am Zool 18:447–460
Neal GE (1970) In: Advances in the Study of the Prostate. Heinemann Medical, London, p 102
Nenci I, Buccati MD, Pifanelli A, Lanza G (1976) Detection and dynamic localization of estradiol receptor complexes in intact target cells by immunofluorescence technique. J Steroid Biochem 7:505–510
O'Malley BW, Buller RE (1975–1976) Current concepts in steroid hormone action. In: Ingbar SH (ed) The Year in Endocrinology. Plenum Medical Book Co, New York, p 277
Pertschuk LP, Tobin EH, Brigati DJ, Gaetjens E (1980) Morphologic assay of steroid hormone receptors in human neoplasia. In: Sommers SC, Rosen PP (eds) Pathology Annual. Appleton-Century Crofts, pp 143–180
Pertschuk LP, Tobin EH, Tanapat P, Gaetjens E, Carter AC, Bloom ND, Macchia RJ, Eisenberg KB (1980) Histochemical analyses of steroid hormone receptors in breast and prostatic carcinoma. J Histochem Cytochem 28:799–810
Pertschuk LP, Rosenthal HE, Macchia RJ, Eisenberg KB, Feldman JG, Wax SH, Kim DS, Whitmore WF Jr, Abrahams JI, Gaetjens E, Wiese GJ, Herr HW, Kerr JP, Murphy GP, Sandberg AA (1982a) Correlation of histochemical and biochemical analyses of androgen binding in prostatic cancer: relation to therapeutic response. Cancer 40:984–993
Pertschuk LP, Tobin EA, Carter AC, Eisenberg KB, Leo VC, Gaetjens E, Bloom ND (1982b) Immunohistologic and histochemical methods for detection of steroid binding in breast cancer. A reappraisal. Breast Cancer Research and Treatment (in press)
Rao BR, Patrick TB, Sweet F (1980) Steroid-albumin conjugate interaction with steroid binding proteins. Endocrinology 106:356–362
Schreibman MP (1964) Studies on the pituitary gland of Xiphophorus maculatus (the platyfish). Zoologica 49:217–243
Schreibman MP, Halpern LR (1980) The demonstration of neurophysin and arginine vasotocin by immunocytochemical methods in the brain and pituitary gland of a teleost fish. Gen Comp Endocrinol 40:1–7
Schreibman MP, Kallman KD (1977) The genetic control of the pituitary gonadal axis in the platyfish, Xiphophorus maculatus. J Exp Zool 200:277–294
Schreibman MP, Kallman KD (1978) The genetic control of sexual maturation in the teleost, Xiphophorus maculatus (Poeciliidae); a review. Ann Biol Anim Biochim Biophys 18:957–962
Schreibman MP, Margolis-Kazan H (1979) Immunocytochemical localization of gonadotropin, its subunits and thyrotropin in the teleost, Xiphophorus maculatus. Gen Comp Endocrinol 39:467–474
Schreibman MP, Leatherland JF, McKeown BA (1973) Functional morphology of the teleost pituitary gland. Am Zool 13:719–742
Schreibman MP, Halpern LR, Goos HJTh, Margolis-Kazan H (1979) Identification of lutenizing hormone-releasing hormone (LH-RH) in the brain and pituitary gland of a fish by immunocytochemistry. J Exp Zool 210:153–160
Schreibman MP, Margolis-Kazan H, Halpern-Sebold L (1982) Immunoreactive gonadotropin and luteinizing hormone releasing hormone in the pituitary gland of neonatal platyfish. Gen Comp Endocrinol 47:385–391
Spelsberg TC, Thrall C, Martin-Dani G, Webster RA, Boyd PA (1979) Steroid receptor interaction with chromatin. In: Hamilton TH, Clark JH, Sadler WA (eds) Ontogeny of receptor and reproductive hormone action. Raven Press, NY, pp 31–63
Szego CM (1974) The lysosome as a mediator of hormone action. Recent Prog Horm Res 30:171–233
Watson GH, Muldoon TG (1977) Microsomal estrogen receptors in rat uterus and anterior pituitary. Fed Proc 36:912
Zänker KS, Prokscha GW, Blümel G (1981) Plasma membrane-integrated estrogen receptors in breast tissue: possible modulator molecules for intercellular hormone level. Cancer Res Clin Oncol 100:135–148
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Schreibman, M.P., Pertschuk, L.P., Rainford, E.A. et al. The histochemical localization of steroid binding sites in the pituitary gland of a teleost (the platyfish). Cell Tissue Res. 226, 523–530 (1982). https://doi.org/10.1007/BF00214781
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DOI: https://doi.org/10.1007/BF00214781