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A morphometric study of the variations in subcellular structures of rat hepatocytes during 24 hours

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Summary

Subcellular structures of hepatocytes in periportal and perivenous zones were examined during 24 h. The volume, surface and numerical profile densities of cytoplasmic organelles were analysed morphometrically.

Most subcellular structures in periportal and perivenous hepatocytes were subject to strong circadian variations. In hepatocytes from both zones, the volume densities of smooth endoplasmic reticulum (sER), mitochondria, lysosomes, peroxisomes, polysomes and lipid droplets demonstrated peak values at 16.00 h, 20.00 h or 00.00 h; trough values were at 04.00 h, 08.00 h, or 12.00 h, except for peroxisomes (16.00 h). However, the volume densities of glycogen granules and rough endoplasmic reticulum (rER) in periportal and perivenous hepatocytes exhibited maximal values at 04.00 h, 08.00 h or 12.00 h and minimal values at 20.00 h. The surface densities of sER, mitochondria, lysosomes and peroxisomes, and the numerical profile densities of mitochondria, lysosomes and peroxisomes in periportal and perivenous hepatocytes showed similar trends. These events suggest that membranes of the rER show a partial correlation with the sER, mitochondrial and lysosomal membranes during the 24-h span. This may involve the interaction between ribosomes and rER. Almost all cytoplasmic organelles examined displayed significant differences between periportal and perivenous hepatocytes, morphometrically and in fine structure, indicating that the morphofunctional variability of hepatocytes differs depending on the location in the liver acinus.

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References

  • Babcock MB, Cardell RR (1975) Fine structure of hepatocytes from fasted and fed rats. Am J Anat 143:399–438

    Google Scholar 

  • Bolender RP, Weibel ER (1973) A morphometric study of the removal of phenobarbital-induced membranes from hepatocytes after cessation of treatment. J Cell Biol 56:746–761

    Google Scholar 

  • Cardell RR (1976) Smooth endoplasmic reticulum in rat hepatocytes during glycogen deposition and depletion. Int Rev Cytol 33:221–279

    Google Scholar 

  • Chedid A, Nair V (1972) Diurnal rhythm in endoplasmic reticulum of rat liver: Electron microscopic study. Science 175:176–179

    Google Scholar 

  • Dallner G, Siekevitz P, Palade GE (1966a) Biogenesis of endoplasmic reticulum membranes. I. Structural and chemical differentiation in developing rat hepatocytes. J Cell Biol 30:73–91

    Google Scholar 

  • Dallner G, Siekevitz P, Palade GE (1966b) Biogenesis of endoplasmic reticulum membranes. II. Synthesis of constitutive microsomal enzymes in developing rat hepatocyte. J Cell Biol 30:97–117

    Google Scholar 

  • Groh V, Mayersbach H v (1981) Enzymatic and functional heterogeneity of lysosomes. Cell Tissue Res 214:613–621

    Google Scholar 

  • Higgins JA, Barrnett RJ (1972) Studies on the biogenesis of smooth endoplasmic reticulum membranes in liver of phenobarbitaltreated rats. I. The site of activity of acyltransferase involved in synthesis of the membrane phospholipid. J Cell Biol 55:282–298

    Google Scholar 

  • Hildebrand J, Thys O, Gérin Y (1973) Alterations of rat liver lysosomes and smooth endoplasmic reticulum induced by the diazafluoranthen derivative AC-3579. II. Effects of the drug on phospholipid metabolism. Lab Invest 28:83–86

    Google Scholar 

  • Jerusalem C, Müller O, Mayersbach H v (1967) Circadian ultrstructural changes in liver cells. In: Mayersbach H v (ed) The cellular aspects of biorhythms. Springer, Berlin-Heidelberg-New York, pp 147–154

    Google Scholar 

  • Jerusalem C, Eling W, Jap P (1970) Histochemische und elektronenmikroskopische Veränderungen der Leberzelle im Tagesrhythmus und unter experimentellen Bedingungen. Acta Histochem 36:168–206

    Google Scholar 

  • Kanamura S, Kubota MA (1980) The heterogeneity of hepatocytes during the postnatal development of the mouse. Anat Embryol 158:151–159

    Google Scholar 

  • Katz N, Teutsch HF, Sasse D, Jungermann K (1977a) Heterogeneous distribution of glucose-6-phosphatase in microdissected periportal and perivenous rat liver tissue. FEBS Lett 76:226–230

    Google Scholar 

  • Katz N, Teutsch HF, Jungermann K, Sasse D (1977b) Heterogeneous reciprocal localization of fructose-1,6-bisphosphatase and of glucokinase in microdissected periportal and perivenous rat liver tissue. FEBS Lett 83:272–276

    Google Scholar 

  • Kubota MA, Kanai K, Kanamura S (1982) Development of ultrastructural heterogeneity among hepatocytes in the mouse. Anat Rec 202:395–405

    Google Scholar 

  • Lazarow PB, de Duve C (1976) A fatty acyl-CoA oxidizing system in rat liver peroxisomes; enhancement by clofibrate, a hypolipidemic drug. Proc Natl Acad Sci USA 73:2043–2046

    Google Scholar 

  • Leskes A, Siekevitz P, Palade GE (1971) Differentiation of endplasmic reticulum in hepatocytes. I. Glucose-6-phosphatase distribution in situ. J Cell Biol 49:264–287

    Google Scholar 

  • Loud AV (1968) A quantitative stereological description of the ultrastructure of normal rat liver parenchymal cells. J Cell Biol 37:27–46

    Google Scholar 

  • Mayersbach H v (1967) Seasonal influences on biological rhythms of standardized laboratory animals. In: Mayersbach H v (ed) The cellular aspects of biorhythms. Springer, Berlin Heidelberg New York, pp 87–99

    Google Scholar 

  • Mayersbach H v (1976) Time — A key in experimental and practical medicine. Arch Toxicol 36:185–216

    Google Scholar 

  • Mayersbach H v, Leske R (1963) Physiologische Schwankungen des Glykogen-Tagesrhythmus. Acta Morphol Acad Sci Hung 7:33–42

    Google Scholar 

  • Montisano D, Cascarano J, Pickett CB (1982) Association between mitochondria and rough endoplasmic reticulum in rat liver. Anat Rec 203:441–450

    Google Scholar 

  • Müller O (1971) Circadianstruktur der Leber. Habilitationsschrift, Medizinische Hochschule Hannover

  • Müller O, Jerusalem C, Mayersbach H v (1966) Die physiologischen Tagesschwankungen in Leberzellen von Ratten. Z Zellforsch 69:438–451

    Google Scholar 

  • Rappaport AM, Borowy ZJ, Lougheed WM, Lotto WN (1954) Subdivision of hexagonal liver lobules into a structural and functional unit. Anat Rec 119:11–33

    Google Scholar 

  • Rieder H (1981) NADP-dependent dehydrogenases in rat liver parenchyma. III. The distribution of a lipogenic area on the basis of histochemically demonstrated enzyme activities and the neutral fat content during fasting and refeeding. Histochemistry 72:579–615

    Google Scholar 

  • Rohr HP, Hundstad A-C, Binachi L, Eckert H (1970) Morphmetrisch-ultrastrukturelle Untersuchungen über die durch die Tageszeit induzierten Veränderungen der Rattenleberparenchymzelle. Acta Anat 76:102–111

    Google Scholar 

  • Sasse D (1975) Dynamics of liver glycogen. The topochemistry of glycogen synthesis, glycogen content and glycogenolysis under the experimental conditions of glycogen accumulation and depletion. Histochemistry 45:237–254

    Google Scholar 

  • Sasse D, Katz N, Jungermann K (1975) Functional heterogeneity of rat liver parenchyma and isolated hepatocytes. FEBS Lett 57:83–88

    Google Scholar 

  • Sasse D, Teutsch HF, Katz N, Jungermann K (1979) The development of functional heterogeneity in the liver parenchyma of the golden hamster. Anat Embryol 156:153–163

    Google Scholar 

  • Sottocasa GL, Kuylenstirna B, Erster L, Bergstrand A (1967a) An electron transport system associated with the outer membrane of liver mitochondria. J Cell Biol 32:415–438

    Google Scholar 

  • Sottocasa GL, Kuylenstirna B, Erster L, Bergstrand A (1967b) Separation and some enzymatic properties of the inner and outer membranes of rat liver mitochondria. Methods Enzymol 10:448–463

    Google Scholar 

  • Stäubli W, Hess R, Weibel ER (1969) Correlated morphometric and biochemical studies on the liver cell. II. Effects of phenobarbital on rat hepatocytes. J Cell Biol 42:92–112

    Google Scholar 

  • Teutsch HF, Rieder H (1979) NADP-dependent dehydrogenases in rat liver parenchyma. II. Comparison of qualitative and quantitative G6PDH distribution patterns with particular reference to sex differences. Histochemistry 60:43–52

    Google Scholar 

  • Uchiyama Y (1980) Chronobiological aspects of cells. Chronologcal changes in the morphology of hepatocytes. Biomed Res 1:376–391

    Google Scholar 

  • Uchiyama Y (1981) Circadian alterations in tubular structures on the outer mitochondrial membrane of rat hepatocytes. Cell Tissue Res 214:519–527

    Google Scholar 

  • Uchiyama Y (1982) The membrane association among the rougand smooth-surfaced endoplasmic reticulum and Golgi apparatus in rat hepatocytes at certain circadian stages. Tohoku J Exp Med 136:299–302

    Google Scholar 

  • Uchiyama Y, Mayersbach H v (1981a) Circadian variations of ultramorphology and glycogen content in hepatocytes of light-manipulated rats. Gegenbaurs Morphol Jahrb 127:452–463

    Google Scholar 

  • Uchiyama Y, Mayersbach H v (1981b) Circadian changes of lysosomal enzyme activities in rat hepatocytes using ultracytochemistry. In: Vidrio EA, Mayersbach H v, Scheving LE, Pauly JE (eds) Biological rhythms in structure and function. Alan R. Liss, New York, pp 117–124

    Google Scholar 

  • Uchiyama Y, Groh V, Mayersbach H v (1981) Different circadian variations as an indicator of heterogeneity of liver lysosomes. Histochemistry 73:321–337

    Google Scholar 

  • Uchiyama Y, Groh V, Mayersbach H v (1982) Circadian changes in thiamine pyrophosphatase activity of rat hepatocytes — A histochemical study at the electron microscopic level. Cell Mol Biol 28:245–254

    Google Scholar 

  • Weibel ER (1979) Stereological methods. Practical methods for biological morphometry. Academic Press, London-New YorkToronto-Sydney-San Francisco

    Google Scholar 

  • Weibel ER, Stäubli W, Gnägi HR, Hess FA (1969) Correlated morphometric and biochemical studies on the liver cell. I. Morphometric model, stereologic methods, and normal morphometric data for rat liver. J Cell Biol 42:68–91

    CAS  PubMed  Google Scholar 

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  1. Department of Anatomy, Institute of Basic Medical Sciences, University of Tsukuba, Niihari-Gun, Ibaraki-Ken, Japan

    Yasuo Uchiyama & Akira Asari

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  1. Yasuo Uchiyama
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  2. Akira Asari
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Uchiyama, Y., Asari, A. A morphometric study of the variations in subcellular structures of rat hepatocytes during 24 hours. Cell Tissue Res. 236, 305–315 (1984). https://doi.org/10.1007/BF00214231

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