Abstract
Cadmium (Cd) inhibited the activities of Na+-K+ ATPase (IC50=5.0×10−5 M), K+-p-nitrophenyl phosphatase (PNPPase) (IC50=4.0×10−5 M) and 3H-ouabain binding (IC50=7.5×10−5 M) in rat brain microsomes. Monothiols (cysteine but not glutathione and D-pencillamine) and dithiols (dimercaprol, dimercaptosuccinic acid and dithiothreitol) offered varied levels of protection against Cd-inhibition of Na+-K+ ATPase. Protection of Na+-K+ ATPase by these sulfhydryl (SH) agents was higher at 7.5 as compared to 8.5 pH. The present data suggest that Cd-inhibited Na+-K+ ATPase, by interfering with phosphorylation of enzyme molecule and dephosphorylation of the enzyme-phosphoryl complex and exerts a similar effect to that of SH-blocking agents.
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Chetty, C.S., Cooper, A., McNeil, C. et al. The effects of cadmium in vitro on adenosine triphosphatase system and protection by thiol reagents in rat brain microsomes. Arch. Environ. Contam. Toxicol. 22, 456–458 (1992). https://doi.org/10.1007/BF00212567
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DOI: https://doi.org/10.1007/BF00212567