Summary
The ultrastructural and cytochemical features of peritoneal mast cells of the rat were studied. Immature mast cells show specific cytoplasmic granules of different sizes, the smaller ones localized in the Golgi region. The rough endoplasmic reticulum and Golgi apparatus are well developed, and mitochondria are numerous. Nuclei show deep indentations. Acid phosphatase is present in the Golgi saccules, in GERL (Golgi apparatus-endoplasmic reticulumlysosome) and in some small granules. It is not present in mature granules. Trimetaphosphatase is present in the Golgi saccules, in GERL, in most immature granules and in some mature granules. These enzymes appear to be transported and packaged into granules by the Golgi apparatus, suggesting that the specific mast cell granules may be a form of lysosome. The results of this study are consistent with the hypothesis that peritoneal mast cells may be derived from macrophage-like precursors.
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References
Bainton DF, Farquhar MG (1968) Differences in enzyme content of azurophil and specific granules of polymorphonuclear leukocytes. II. Cytochemistry and electron microscopy of bone marrow cells. J Cell Biol 39:299–317
Bainton DF, Farquhar MG (1970) Segregation and packaging of granule enzymes in eosinophilic leukocytes. J Cell Biol 45:54–73
Bentfeld-Barker ME, Bainton DF (1980) Cytochemical localization of arylsulfatase B in rat basophils and mast cells. J Histochem Cytochem 28:1055–1061
Bloom GD (1974) Structural and biochemical characteristics of mast cells. In: Zweifach BW, Grant L, McCluskey RT (eds) The inflammatory process, Vol. II. Academic Press, New York, pp 545–599
Clausen J, Jahn H, Nielsen EH (1983) Electron microscopical study of rat mast cell maturation. Virchows Arch (Cell Pathol) 43:151–158
Cohn ZA, Fedorko ME, Hirsch JG (1966) The in vitro differentiation of mononuclear phagocytes. V. The formation of phagocyte lysosomes. J Exp Med 123:757–766
Combs JW (1966) Maturation of rat mast cells. An electron microscope study. J Cell Biol 31:563–575
Czarnetzki BM, Behrendt H (1981) Studies on the in vitro development of rat peritoneal mast cells. Immunobiology 159:256–268
Czarnetzki BM, Hannich D, Niedorf H (1979) In-vitro studies on the development of rat peritoneal mast cells. Immunobiology 156:470–476
Czarnetzki BM, Sterry W, Bazin H, Kalveram KJ (1982) Evidence that tissue mast cells derive from mononuclear phagocytes. Int Arch Allergy Appl Immunol 67:44–48
Daems WT (1980) Peritoneal macrophages. In: Carr I, Daems WT (eds) The reticuloendothelial system. A comprehensive treatise, Vol 1, Morphology. Plenum Press, New York, London, pp 57–127
Denburg JA, Richardson M, Telizyn S, Bienenstock J (1983) Basophil/mast cell precursors in human peripheral blood. Blood 61:775–780
Doty SB, Smith CE, Hand AR, Oliver C (1977) Inorganic trimetaphosphatase as a histochemical marker for lysosomes in light and electron microscopy. J Histochem Cytochem 25:1381–1384
Essner E (1973) Phosphatases. In: Hayat MA (ed) Electron microscopy of enzymes. Vol 1. Van Nostrand Reinhold Company, New York, pp 44–76
Fawcett DW (1954) Cytological and pharmacological observations on the release of histamine by mast cells. J Exp Med 100:217–224
Galli SJ, Dvorak AM, Dvorak HF (1984) Basophils and mast cells: Morphologic insights into their biology, secretory patterns, and function. In: Ishizaka K (ed) Mast cell activation and mediator release. Prog Allergy, Vol 34. Karger, Basel, pp 1–141
Gomori G (1952) Microscopic histochemistry: principles and practice, University Chicago Press, Chicago, pp 189–194
Hand AR, Oliver C (1977) Cytochemical studies of GERL and its role in secretory granule formation in exocrine cells. Histochem J 9:375–392
Hand AR, Oliver C (1984) The role of GERL in the secretory process. In: Cantin M (ed) Cell biology of the secretory process. Karger, Basel, pp 148–170
Kitamura Y, Miyoshi I (1978) 7. Development and differentiation of mast cells and basophils. Acta Haematol Jpn 41:1251–1259
Kitamura Y, Shimada M, Hatanaka K, Miyano Y (1977) Development of mast cells from grafted bone marrow in irradiated mice. Nature 268:442–443
Komiyama A, Spicer SS (1975) Acid phosphatase demonstrated ultrastructurally in mast cell granules altered by pinocytosis. Lab Invest 32:485–491
Lawson D, Raff MC, Gomperts B, Fewtrell C, Gilula NB (1977) Molecular events during membrane fusion. A study of exocytosis in rat peritoneal mast cells. J Cell Biol 72:242–259
Martin TW, Lagunoff D (1984) Mast cell secretion. In: Cantin M (ed) Cell biology of the secretory process. Karger, Basel, pp 481–516
Mendonca VO, Vugman I (1982) Maturacão e regeneracão de mastócitos peritoneais e do mesentério de rato. Influencia de drenagem linfática. III Congresso Brasileiro de Biologia Celular, São Paulo
Metcalfe DD, Donlon MA, Kaliner M (1981) The mast cell. CRC Crit Rev Immunol 3:23–74
Nichols BA (1976) Normal rabbit alveolar macrophages. II. Their primary and secondary lysosomes as revealed by electron microscopy and cytochemistry. J Exp Med 144:920–932
Nichols BA, Bainton DF (1973) Differentiation of human monocytes in bone marrow and blood. Sequential formation of two granule populations. Lab Invest 29:27–40
Nichols BA, Bainton DF, Farquhar MG (1971) Differentiation of monocytes. Origin, nature, and fate of their azurophil granules. J Cell Biol 50:498–515
Novikoff AB (1963) Lysosomes in the physiology an pathology of cells: Contributions of staining methods. In: de Reuck AVS, Cameron MP (eds) Ciba Foundation Symposium on Lysosomes. Little Brown and Co, Boston, pp 36–73
Novikoff AB (1976) The endoplasmic reticulum: a cytochemist's view (a review). Proc Natl Acad Sci USA 73:2781–2787
Padawer J (1969) Uptake of colloidal thorium dioxide by mast cells. J Cell Biol 40:747–760
Padawer J (1978) The mast cell and immediate hypersensitivity. In: Bach MK (ed) Immediate hypersensitivity, Modern concepts and developments. Marcel Dekker, New York and Basel, pp 301–367
Parwaresch MR, Müller-Hermelink HK, Desaga JF, Zakari V, Lennert K (1971) Die Herkunft der Gewebsmastzellen bei der Ratte, zugleich ein Beitrag zur quantitativen Cytologie der sterilen Peritonitis. Virchows Arch Abt B Zellpath 8:20–35
Robinson JM, Karnovsky MJ (1983) Ultrastructural localization of several phosphatases with cerium. J Histochem Cytochem 31:1197–1208
Sannes PL, Spicer SS (1979) The heterophagic granules of mast cells. Dipeptidyl aminopeptidase II activity and resistance to exocytosis. Am J Pathol 94:447–458
Sannes PL, McDonald JK, Allen RC, Spicer SS (1979) Cytochemical localization and biochemical characterization of dipeptidyl aminopeptidase II in macrophages and mast cells. J Histochem Cytochem 27:1496–1498
Schwartz LB, Austen KF (1980) Enzymes of the mast cell granule. J Invest Dermatol 74:349–353
Schwartz LB, Austen KF (1984) Structure and function of the chemical mediators of mast cells. In: Ishizaka K (ed) Mast cell activation and mediator release. Prog Allergy, Vol 34. Karger, Basel, pp 271–321
Spurr AR (1969) A low viscosity epoxy resin embedding medium for electron microscopy. J Ultrastruct Res 26:31–43
Sterry W, Czarnetzki BM (1982) In vitro differentiation of rat peritoneal macrophages into mast cells: an enzyme cytochemical study. Blut 44:211–220
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Jamur, M.C., Vugman, I. & Hand, A.R. Ultrastructural and cytochemical studies of acid phosphatase and trimetaphosphatase in rat peritoneal mast cells developing in vivo. Cell Tissue Res. 244, 557–563 (1986). https://doi.org/10.1007/BF00212533
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DOI: https://doi.org/10.1007/BF00212533