Abstract
HuLy-m3 is an M r 47 000 pan-leukocyte antigen detected by the monoclonal antibody (mAb) 5-4.8. This report describes the isolation and analysis of a cDNA clone encoding HuLy-m3. Serological analysis demonstrated that antibodies of the CD48 cluster also reacted with transfected cells expressing HuLy-m3. The DNA sequence of the clone suggests linkage to the cell membrane through a glycosyl phosphatidylinositol tail and this was verified experimentally. Sequence similarity with the human B-cell activation antigen Blast-1 was noted.
Similar content being viewed by others
References
Aruffo, A. and Seed, B.: Molecular cloning of a CD28 cDNA by a high-efficiency COS cell expression system. Proc Natl Acad Sci USA 84: 8573–8577, 1987
Fischer, R. C., Lebeau, M. M., Laurence, J. B., and Thorley-Lawson, D. A.: Genomic organization and chromosomal localization of the human leukocyte activation antigen BLAST-1. In W. Knapp (ed.): “Leukocyte Typing IV”, Oxford University Press, Oxford, 1989
Gilman, M.: Preparation and analysis of RNA. In F. M. Ausubel (ed.): Current Protocols in Molecular Biology, pp. 4.1.1–4.1.6, Wiley, New York, 1989
Goding, J. W.: Immunoprecipitations and electrophoretic analysis of membrane proteins. In J. C. Venter and L. C. Harrison (eds.): Membrane, Receptor Purification and Characterisation Techniques, pp. 31–60, Luiss, New York, 1980
Henniker, A. J., Bradstock, K. F., Atkinson, K., Barclay, S., Kabral, A., and Grimsley, P.: Expression of a novel human pan leukocyte differentiation antigen on leukaemic cells. Leuk Res 13: 689–697, 1989
Henniker, A. J., Bradstock, K. F., Grimsley, P., and Atkinson, K.: A novel human leukocyte surface membrane antigen defined by murine monoclonal antibody. Tissue Antigens, in press, 1990
Killeen, N., Moessner, R., Arvieux, J., Willis, A., and Williams, A. F.: The MRC OX-45 antigen of rat leukocytes and endothelium is in a subset of the immunoglobulin superfamily with CD2, LFA-3 and carcinoembryonic antigens. EMBO J 7: 3087–3091, 1988
Knapp, W. (ed.): Leukocyte Typing IV, Oxford University Press, Oxford, 1989
McMichael, A. J. (ed.): Leukocyte Typing III, Oxford University Press, Oxford, 1987
Parish, C. R. and McKenzie, I. F. C.: A sensitive rosetting method for detecting subpopulations of lymphocytes which react with alloantisera. J Immunol Methods 20: 173–183, 1978
Seed, B. and Aruffo, A.: Molecular cloning of the CD2 antigen, the T-cell erythrocyte receptor by a rapid immuno selection procedure. Proc Natl Acad Sci USA 84: 3365–3369, 1987
Sparrow, R. L.: Human antigen defined by monoclonal antibodies. Ph. D. thesis, University of Melbourne, Parkville, Australia, 1983
Sparrow, R. L. and McKenzie, I. F. C.: A function for human T200 in natural killer cytolysis. Transplantation 36: 166–171, 1983
Starling, G. C., Davidson, S. E., McKenzie, J. C., and Hart, D. N. J.: Inhibition of natural killer cell mediated cytolysis with monoclonal antibodies to restricted and non restricted epitopes of the leukocyte common antigen. Immunology 61: 351–356, 1987
Staunton, D. E. and Thorley-Lawson, D. A.: Molecular cloning of the lymphocyte activation marker Blast 1. EMBO J 6: 3695–3701, 1987
Staunton, D. E., Fischer, R. C., LeBeau, M. M., Lawrence, J. B., Barton, D. E., Francke, U., Dustin, M., and Thorley-Lawson, D. A.: Blast-1 possesses a glycosyl-phosphatidylinositol (GPI) membrane anchor, is related to LFA-3 and OX45, and maps to chromosome 1q21–23. J Exp Med 169: 1087–1099, 1989
Thorley-Lawson, D. A., Schooley, R. T., Bhan, A. K., and Nadler, L. M.: Epstein-Barr virus superinduces a new human B cell differentiation antigen (BLast 1) expressed on transformed lymphoblasts. Cell 30: 415–425, 1982
Thurlow, P. J., Lovering, K. E., and McKenzie, I. F. C.: A monoclonal antibody detecting a new human T cell antigen, HuLy-m2. Transplantation 38: 143–147, 1984
Vaughan, H. A., Thompson, C. H., Sparrow, R. L., and McKenzie, I. F. C.: HuLy-m3—a human leukocyte antigen. Transplantation 36: 446–450, 1983
Author information
Authors and Affiliations
Additional information
The nucleotide sequence data reported in this paper have been submitted to the GenBank nucleotide sequence database and have been assigned the accession number M 59904.
Rights and permissions
About this article
Cite this article
Vaughan, H.A., Henning, M.M., Purcell, D.F.J. et al. The isolation of cDNA clones for CD48. Immunogenetics 33, 113–117 (1991). https://doi.org/10.1007/BF00210824
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00210824